Mycoplasma pneumoniae host-pathogen studies in an air-liquid culture of differentiated human airway epithelial cells
Mycoplasma pneumoniae host-pathogen studies in an air-liquid culture of differentiated human airway epithelial cells
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DOI：
10.1016/j.micpath.2006.11.003
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Krause, Duncan C.
中科院分区：
文献类型：
--
作者：
Krunkosky, Thomas M.;Jordan, Jarrat L.;Krause, Duncan C.
体内肺炎支原体与气道上皮之间的相互作用是复杂和多方面的。虽然已经进行了多项体外研究，研究了这种与细胞系、动物细胞和器官培养模型的相互作用，但在气液界面培养中，肺炎支原体与完全分化的人气道上皮之间的相互作用仍未被探索。在本研究中，我们利用分化的正常人支气管上皮细胞（NHBE）的气液界面培养研究了肺炎支原体与气道上皮的相互作用。利用共聚焦显微镜，我们发现肺炎支原体细胞最初与纤毛上皮细胞结合，但随着时间的推移，定植变得更均匀地分布在整个表面。在该培养体系中，肺炎支原体感染刺激细胞间粘附分子I （ICAM-1）基因表达和可溶性ICAM-1的产生。(c) 2006 Elsevier Ltd.版权所有。
The interaction between Mycoplasma pneumonaie and the airway epithelium in vivo is complex and multifaceted. While multiple in vitro studies have been conducted studying this interaction with cell lines and animal cell and organ culture models, the interactions between M. pneumoniae and fully differentiated human airway epithelium in air liquid interface culture remains unexplored. In the present study we investigated M. pneumoniae interactions with airway epithelium utilizing an air-liquid interface culture of differentiated normal human bronchial epithelial (NHBE) cells. Utilizing confocal microscopy we found that M. pneumoniae cells bound initially to ciliated epithelial cells, but colonization became more evenly distributed over the entire surface with time. M. pneumoniae infection resulted in stimulation of intercellular adhesion molecule I (ICAM-1) gene expression and soluble ICAM-1 production in this culture system. (c) 2006 Elsevier Ltd. All rights reserved.