Boosted photoelectrochemical immunosensing of metronidazole in tablet using coral-like g-C3N4 nanoarchitectures
Boosted photoelectrochemical immunosensing of metronidazole in tablet using coral-like g-C3N4 nanoarchitectures
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使用珊瑚状 g-C3N4 纳米结构增强片剂中甲硝唑的光电化学免疫传感
DOI：
10.1016/j.bios.2018.09.084
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发表时间：
2019-01-01
影响因子：
12.6
通讯作者：
Gong, Jingming
中科院分区：
文献类型：
--
作者：
Li, Xin;Yuan, Yijin;Gong, Jingming
提出了一种在可见光照射下检测常用口服药物中甲硝唑（MNZ）的光电化学（PEC）生物检测方法，首次探索了新型分级珊瑚状g-C3 N4纳米结构（cg-C3 N4）作为PEC传感平台。具有独特的纳米结构（例如，由于cgC（3）N（4）纳米结构具有交错的多孔网络结构和开放的边界），因此所形成的cgC（3）N（4）纳米结构不仅有效地抑制了光生电子-空穴的复合，而且能够使捕获抗体的固定以及抗体-抗原结合效率流畅，从而放大了光电流响应。这种新构建的PEC免疫分析法显示出良好的性能，MNZ的测定具有高灵敏度和高选择性。在最佳条件下，该生物测定方法在信噪比为3时，线性范围为0.01-100 μ M，检测限为0.005 μ M。结果表明，PEC免疫分析法可用于检测常见口腔药物样品中的MNZ。
A simple, facile and sensitive photoelectrochemical (PEC) bioassay protocol for metronidazole (MNZ) detection in common oral medicine samples has been proposed under visible-light irradiation, where novel hierarchical coral-like g-C3N4 nanoarchitectures (cg-C3N4) have been first explored as PEC sensing platform. Featured with the unique nanostructures (e.g., interlaced porous network architecture, and open boundaries), the as-formed cgC(3)N(4) nanoarchitectures not only efficiently inhibit the recombination of photogenerated electron-hole but also enable the immobilization of capture antibodies as well as the antibody-antigen binding efficiency fluently, thus amplifying the photocurrent response. This newly constructed PEC immunoassay displays excellent performance for MNZ determination with high sensitivity and selectivity. Under the optimal condition, this bioassay protocol exhibits a linear range of 0.01-100 mu M with a detection limit of 0.005 mu M at signal to noise ratio of 3. The resulting PEC immunoassay has been proved to be applicable for sensing MNZ in common oral medicine samples.