Genomic analysis reveals the biosynthesis pathways of diverse secondary metabolites and pinoresinol and its glycoside derivatives in Phomopsis sp.XP-8
Genomic analysis reveals the biosynthesis pathways of diverse secondary metabolites and pinoresinol and its glycoside derivatives in Phomopsis sp.XP-8
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DOI：
10.13343/j.cnki.wsxb.20170604
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
Zhenhong Gao;Zhiwei Zhang;Xiaoguang Xu;J. Che;Yan Zhang;Yanlin Liu;Junling Shi
中科院分区：
文献类型：
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作者：
Zhenhong Gao;Zhiwei Zhang;Xiaoguang Xu;J. Che;Yan Zhang;Yanlin Liu;Junling Shi
目的对拟南星属(Phomopsis sp.)XP-8基因组有助于揭示该菌株潜在的代谢途径，以及与松脂醇及其糖苷衍生物和其他次生代谢物生物合成相关的关键基因。方法对拟南芥进行了测序。XP-8基因组采用Illumina HiSeq 2500高通量测序平台。然后使用不同的软件对基因预测和功能注释进行分析。结果最终组装的基因组大小约为55.2Mb，总GC含量为53.5%。进一步的注释分析预测了17094个蛋白质编码基因和310个非编码基因。确定了一大组与松脂醇及其糖苷衍生物和其他次生代谢物的产生有关的候选基因。正交学和系统发育分析表明，拟南星属Phomopsis sp.XP-8和子囊菌5共有12635个同源基因和5626个基因家族。结论拟南星属Phomopsis sp.XP-8菌株具有产生松脂醇及其糖苷衍生物等多种次生代谢物的基因组基础。本研究为进一步的松脂醇代谢工程及其糖苷生产提供了依据。
ObjectiveSequencing and analysis of Phomopsis sp. XP-8 genome are beneficial to reveal the potential metabolic pathways of this strain and the key genes related to the biosynthesis of pinoresinol and its glycoside derivatives and other secondary metabolites. MethodsWe sequenced Phomopsis sp. XP-8 genome by the Illumina HiSeq 2500 high throughput sequencing platform. Then gene prediction and functional annotation were analyzed using different softwares. ResultsThe final assembled genome size was approximately 55.2 Mb with an overall GC content of 53.5%. Further annotation analyses predicted 17094 protein-coding genes and 310 non-coding RNA genes. A large set of candidate genes involved in the production of pinoresinol, its glycoside derivatives and other secondary metabolites were identified. Orthology and phylogenetic analysis revealed that Phomopsis sp. XP-8 and 5 Ascomycota share 12635 orthologous genes and 5626 gene families. ConclusionPhomopsis sp. XP-8 possessed genomic basis for production of diverse secondary metabolites, including pinoresinol and its glycoside derivatives. This study provides basis for the further metabolic engineering of pinoresinol and its glucoside production.