Thymidine kinase-1/CD31 double immunostaining for identifying activated tumor vessels
Thymidine kinase-1/CD31 double immunostaining for identifying activated tumor vessels
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DOI：
10.1080/10520295.2018.1499962
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发表时间：
2018-10
影响因子：
1.6
通讯作者：
Shu Okamura;T. Osaki;K. Nishimura;Hiroyuki Ohsaki;Michiko Shintani;Hiroshi Matsuoka;Kotaro Maeda;Kazuya Shiogama;Tomoo Itoh;Shingo Kamoshida
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文献类型：
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作者：
Shu Okamura;T. Osaki;K. Nishimura;Hiroyuki Ohsaki;Michiko Shintani;Hiroshi Matsuoka;Kotaro Maeda;Kazuya Shiogama;Tomoo Itoh;Shingo Kamoshida
摘要虽然血管生成在肿瘤的生长和发展中起着至关重要的作用，但目前还没有可靠的方法来评估肿瘤组织切片中的血管生成。使用对增殖内皮细胞具有高度特异性的生物标志物可以帮助量化血管生成活性。胸苷激酶-1（TK 1）是一种参与DNA合成补救途径的酶，其活性与细胞增殖相关。我们研究了使用TK 1和CD 31双重免疫染色来识别活化的肿瘤血管。对39例结直肠癌组织中TK 1/CD 31阳性血管率（PVRs）与癌旁正常组织进行比较，并与Ki 67/CD 31双染组织进行比较。CRC中的平均TK 1/CD 31 PVR（23.6%）是邻近正常组织（1.7%）的13.9倍。相比之下，CRC中的平均Ki 67/CD 31 PVR为20.0%，即仅为正常组织（4.2%）的4.8倍。此外，正常组织中的平均TK 1/CD 31 PVR显著低于平均Ki 67/CD 31 PVR。我们的研究结果表明，TK 1/CD 31的双重免疫染色可以比Ki 67/CD 31染色更准确地检测活化的肿瘤血管，并可能识别对抗血管生成治疗有反应的肿瘤。
ABSTRACT Although angiogenesis plays a crucial role in cancer growth and progression, no reliable method for assessing angiogenesis in tumor tissue sections currently is available. Using biomarkers with high specificity for proliferating endothelial cells could help quantify angiogenic activity. Thymidine kinase-1 (TK1) is an enzyme involved in the salvage pathway of DNA synthesis and its activity is correlated with cell proliferation. We investigated the use of double immunostaining for TK1 and CD31 for identifying activated tumor vessels. Differences in TK1/CD31 positive vessel rates (PVRs) between tumor and adjacent normal tissues were evaluated in 39 colorectal carcinoma (CRC) samples and compared with those of Ki67/CD31 double stained tissues. Mean TK1/CD31 PVR (23.6%) in CRCs was 13.9 fold greater than in adjacent normal tissues (1.7%)). By comparison, mean Ki67/CD31 PVR in CRCs was 20.0%, i.e. only 4.8 fold greater than in normal tissues (4.2%). Also, mean TK1/CD31 PVR in normal tissues was significantly less than mean Ki67/CD31 PVR. Our findings indicate that double immunostaining for TK1/CD31 can detect activated tumor vessels more accurately than staining for Ki67/CD31 and potentially could identify tumors that will respond to anti-angiogenic therapy.