Detection of mRNAs containing regulatory peptide coding sequences using synthetic oligodeoxynucleotides.
Detection of mRNAs containing regulatory peptide coding sequences using synthetic oligodeoxynucleotides.
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使用合成寡脱氧核苷酸检测含有调节肽编码序列的 mRNA。
DOI：
10.1002/jcb.240260303
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发表时间：
1984
影响因子：
4
通讯作者：
Fridkin,M
中科院分区：
文献类型：
--
作者：
Gozes,I;Bodner,M;Shani,Y;Fridkin,M
为了了解肽类激素产生的调节，阐明它们的生物合成途径至关重要。我们选择研究一种主要的调节肽，血管活性肠肽（VIP），一种具有神经递质和神经激素作用的肽。为了鉴定特定的肽mRNA，我们使用放射性标记的合成寡聚氧核苷酸作为杂交探针，其序列与预测的肽mRNA序列互补。采用这种方法，我们鉴定并部分纯化了一种含有VIP相关序列的1600个碱基长的mRNA，该序列可以在体外被重组为VIP免疫反应性多肽。在正常的VIP产生组织（大鼠脑）以及产生VIP的肿瘤（人口腔肿瘤）中检测到这种mRNA。这种mRNA的大小与在人类神经母细胞瘤细胞中鉴定的已知VIP-mRNA不同，这表明不同细胞类型中存在不同VIP-mRNA的可能性。
To understand the regulation of the production of peptide hormones, it is vital to elucidate their biosynthetic pathways. We chose to study a major regulatory peptide, vasoactive intestinal peptide (VIP), a peptide possessing both neurotransmitter and neurohormone actions. To identify the specific peptide mRNA we are using, as hybridization probes, radiolabeled synthetic oligodcoxynucleotides with sequence complementary to the predicted peptide mRNA sequence. Employing this approach, we identified and partially purified a ∼ 1600‐base long mRNA containing VIP related sequences which can be translatedin vitrointo VIP‐immunoreactive polypeptides. Such mRNA was detected in normal VIP producing tissue (rat brain), as well as in a tumor producing VIP (human buccal tumor). This mRNA differs in size from a known VIP‐mRNA identified in human neuro‐blastoma cells, suggesting the possibility of different VIP‐mRNAs in different cell types.