3‐Hz subthreshold oscillations of CA2 neurons In vivo
3‐Hz subthreshold oscillations of CA2 neurons In vivo
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DOI：
10.1002/hipo.22657
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发表时间：
2016-09
期刊：
影响因子：
3.5
通讯作者：
N. Matsumoto;Kazuki Okamoto;Yu Takagi;Y. Ikegaya
中科院分区：
文献类型：
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作者：
N. Matsumoto;Kazuki Okamoto;Yu Takagi;Y. Ikegaya
CA 2区在海马体中是独特的;它接受来自几个下丘脑核团的直接突触神经支配，并表达各种神经调质受体，包括腺苷、加压素和催产素。此外，CA 2区可能具有不同的脑功能，如本能和社会行为的控制;然而，对体内CA 2神经元阈下膜电位的动态知之甚少。我们对乌拉坦麻醉小鼠的CA 2锥体细胞进行了全细胞电流钳记录，并监测了其膜电位的内在波动。CA 2锥体细胞以平均0.8Hz的放电率发出自发动作电位。在大约一半的神经元中，阈下膜电位振荡频率为0.3Hz。在两个神经元中，我们获得了来自CA 1放射层的局部场电位的同时记录，并证明了CA 2神经元的3-Hz振荡与CA 1场电位无关。在河豚毒素灌注的急性海马脑片中，CA 2锥体细胞的膜电位并不优先被3 Hz正弦电流输入所吸引，这表明细胞内3 Hz振荡反映了周围网络的神经元动力学。© 2016 Wiley Periodicals，Inc.
The CA2 region is unique in the hippocampus; it receives direct synaptic innervations from several hypothalamic nuclei and expresses various receptors of neuromodulators, including adenosine, vasopressin, and oxytocin. Furthermore, the CA2 region may have distinct brain functions, such as the control of instinctive and social behaviors; however, little is known about the dynamics of the subthreshold membrane potentials of CA2 neurons in vivo. We conducted whole‐cell current‐clamp recordings from CA2 pyramidal cells in urethane‐anesthetized mice and monitored the intrinsic fluctuations in their membrane potentials. The CA2 pyramidal cells emitted spontaneous action potentials at mean firing rates of ∼0.8 Hz. In approximately half of the neurons, the subthreshold membrane potential oscillated at ∼3 Hz. In two neurons, we obtained simultaneous recordings of local field potentials from the CA1 stratum radiatum and demonstrated that the 3‐Hz oscillations of CA2 neurons were not correlated with CA1 field potentials. In tetrodotoxin‐perfused acute hippocampal slices, the membrane potentials of CA2 pyramidal cells were not preferentially entrained to 3‐Hz sinusoidal current inputs, which suggest that intracellular 3‐Hz oscillations reflect the neuronal dynamics of the surrounding networks. © 2016 Wiley Periodicals, Inc.