Isolation and Characterisation of a Ferrirhodin Synthetase Gene from the Sugarcane Pathogen Fusarium sacchari
Isolation and Characterisation of a Ferrirhodin Synthetase Gene from the Sugarcane Pathogen Fusarium sacchari
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DOI：
10.1002/cbic.201200587
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发表时间：
2013-02-11
期刊：
影响因子：
3.2
通讯作者：
Lazarus, Colin M.
中科院分区：
文献类型：
--
作者：
Munawar, Asifa;Marshall, James W.;Lazarus, Colin M.
FSN 1基因是从甘蔗病原菌Fusarium sacchari中分离得到的一个基因，编码一个4707个氨基酸残基的非核糖体肽合成酶，由三个完整的腺苷酸化、巯基化和缩合模块以及两个额外的巯基化和缩合结构域重复序列组成。这种结构与铁微菌素合成酶的结构相似，铁微菌素合成酶是一种铁载体，在其他丝状真菌中参与细胞内铁的储存。FSN1在曲霉中的异源表达导致分泌的代谢产物的积累，该代谢产物被鉴定为红铁蛋白。这种铁载体存在于F. sacchari的铁微菌素，而铁微菌素只存在于菌丝体中，表明铁微菌素是F.蔗糖，而血红蛋白用于铁的获取。据我们所知，这是第一个报道，在功能上的红铁蛋白合成酶基因。
FSN1, a gene isolated from the sugar-cane pathogen Fusarium sacchari, encodes a 4707-residue nonribosomal peptide synthetase consisting of three complete adenylation, thiolation and condensation modules followed by two additional thiolation and condensation domain repeats. This structure is similar to that of ferricrocin synthetase, which makes a siderophore that is involved in intracellular iron storage in other filamentous fungi. Heterologous expression of FSN1 in Aspergillus oryzae resulted in the accumulation of a secreted metabolite that was identified as ferrirhodin. This siderophore was found to be present in both mycelium and culture filtrates of F. sacchari, whereas ferricrocin is found only in the mycelium, thus suggesting that ferricrocin is an intracellular storage siderophore in F. sacchari, whereas ferrirhodin is used for iron acquisition. To our knowledge, this is the first report to characterise a ferrirhodin synthetase gene functionally.