Overexpression of the PeaT1 Elicitor Gene from Alternaria tenuissima Improves Drought Tolerance in Rice Plants via Interaction with a Myo-Inositol Oxygenase.
Overexpression of the PeaT1 Elicitor Gene from Alternaria tenuissima Improves Drought Tolerance in Rice Plants via Interaction with a Myo-Inositol Oxygenase.
复制标题
来自 Alternaria tenuissima 的 PeaT1 Elicitor 基因的过表达通过与肌醇氧化酶的相互作用提高了水稻的耐旱性
DOI：
10.3389/fpls.2017.00970
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发表时间：
2017
影响因子：
5.6
通讯作者：
Qiu D
中科院分区：
文献类型：
--
作者：
Shi F;Dong Y;Zhang Y;Yang X;Qiu D
非生物胁迫，特别是干旱，严重威胁着谷类作物的产量和品质。在这项研究中，我们观察到过表达Alternariatenuissima PeaT 1基因的水稻植株表现出增强的干旱胁迫耐受性，并提高了干旱处理后的存活率。在PeaT 1-overexpressing（PeaT 1 OE）的植物，脱落酸和叶绿素含量显着增加，而丙二醛（MDA）含量降低与野生型植物相比。此外，我们证实，包括OsAM 1、OsLP 2和OsDST在内的干旱响应基因的转录水平在PeaT 1 OE植物中显着较低。与此相反，在PeaT 1 OE植物中，编码正干旱胁迫调节因子的基因包括OsSKIPa、OsCPK 9、OsNAC 9、OSEREBP 1和OsTPKb的表达水平上调。结合酵母双杂交实验，发现PeaT 1与肌肌醇加氧酶（OsMIOX）存在相互作用，并通过pull-down实验验证了这一点。有趣的是，OsMIOX在干旱处理期间在PeaTlOE植物中高表达。此外，OsMIOX-GFP融合蛋白在烟草原生质体中与内质网（ER）标记共定位，表明OsMIOX在ER中发挥作用。因此，我们的研究结果有助于阐明PeaT 1提高耐旱性的分子机制。
Abiotic stresses, especially drought, seriously threaten cereal crops yields and quality. In this study, we observed that the rice plants of overexpression the Alternariatenuissima PeaT1 gene showed enhanced drought stress tolerance and increased the survival rate following a drought treatment. In PeaT1-overexpressing (PeaT1OE) plants, abscisic acid and chlorophyll content significantly increased, while the malondialdehyde (MDA) content decreased compared with the wild-type plants. Additionally, we confirmed that the transcript levels of drought-responsive genes, including OsAM1, OsLP2, and OsDST, were prominently lower in the PeaT1OE plants. In contrast, expression levels of genes encoding positive drought stress regulators including OsSKIPa, OsCPK9, OsNAC9, OSEREBP1, and OsTPKb were upregulated in PeaT1OE plants. Furthermore, combing the yeast two-hybrid assay, we found that PeaT1 could interact with amyo-inositol oxygenase (OsMIOX), which was verified by pull-down assay. Interestingly, OsMIOX was highly expressed in PeaT1OE plants during the drought treatment. Additionally, the OsMIOX-GFP fusion protein co-localized with the endoplasmic reticulum (ER) marker in tobacco protoplasts, suggesting OsMIOX performs its function in ER. Therefore, our results are useful for elucidating the molecular mechanism underlying the improvement of drought tolerance by PeaT1.