Binding of human natural antibodies to nonalphaGal xenoantigens on porcine erythrocytes.
Binding of human natural antibodies to nonalphaGal xenoantigens on porcine erythrocytes.
复制标题
人类天然抗体与猪红细胞上的非αGal异种抗原的结合。
DOI：
10.1097/00007890-200006150-00036
复制
发表时间：
2000
期刊：
影响因子：
6.2
通讯作者：
Zhu,A
中科院分区：
文献类型：
--
作者：
Zhu,A
背景：异种移植被认为是解决移植器官和细胞严重短缺的可能解决方案之一。尽管 αGal 表位（Galα1、3Galβ1、4GlcNAc-R）已被确定为导致超急性排斥反应的主要异种抗原，但仅从人血清中去除抗 αGal 抗体不足以规避抗体介导的免疫反应。方法和结果。我们在此报告针对不含 αGal 表位（非 αGal 异种抗原）的抗原的异种反应性人类天然抗体的表征使用流式细胞术研究猪红细胞及其参与补体介导的溶血的证据。此外，从猪红细胞膜中分离出一种 45-kDa 的新蛋白，作为人抗非αGal 识别的主要蛋白抗原。结论。这里提供的数据将增加我们对猪红细胞异种抗原的了解，并且对于制定生产与人类免疫相容的修饰红细胞的策略非常重要。
Background.Xenotransplantation is considered one of possible solutions for the serious shortage of organs and cells in transplantation. Although the αGal epitope (Galα1, 3Galβ1, 4GlcNAc-R) has been identified as being a major xenoantigen responsible for hyperacute rejection, the removal of anti-αGal antibody alone from human serum is insufficient to circumvent antibody-mediated immune responses.Methods and Results.We report here the characterization of xenoreactive human natural antibodies against antigens without the αGal epitope (nonαGal xenoantigens) on porcine erythrocytes using flow cytometry and the evidence for their involvement in complement-mediated hemolysis. Furthermore, a novel protein of 45-kDa has been isolated from the porcine erythrocyte membrane as a major protein antigen recognized by human anti-nonαGal.Conclusion.The data presented here will add to our knowledge of xenoantigens on porcine red cells and be important for developing strategies to produce modified red cells immunologically compatible to humans.