Human antigen-presenting cells respond differently to gut-derived probiotic bacteria but mediate similar strain-dependent NK and T cell activation
Human antigen-presenting cells respond differently to gut-derived probiotic bacteria but mediate similar strain-dependent NK and T cell activation
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DOI：
10.1111/j.1574-695x.2007.00333.x
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Frokiaer, Hanne
中科院分区：
文献类型：
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作者：
Fink, Lisbeth N.;Zeuthen, Louise H.;Frokiaer, Hanne
肠道微生物群对于局部和全身免疫系统的稳态是必不可少的，并且特别是乳酸菌和大肠杆菌的菌株已经显示出对炎症性病症如过敏和炎症性肠病具有平衡作用。然而，不同菌株的免疫调节作用的体外评估可能在很大程度上取决于用作模型的细胞类型。为了选择用于筛选人体细胞中有益细菌的最合适的模型，比较了三种类型的抗原呈递细胞（APC）对肠道细菌菌株的应答;血髓样树突状细胞（DC）、单核细胞衍生的DC和单核细胞，并且表征了自然杀伤细胞和幼稚T细胞的效应器应答。肠源性细菌诱导的成熟在APC之间不同，血液DC和单核细胞对细菌产生IL-6和肿瘤坏死因子-α作出反应，这主要引起单核细胞源性DC中的IL-10。相比之下，在自然杀伤细胞和由所有细菌成熟的APC诱导的T细胞中发现了可比较的IFN-γ产生模式。某些菌株对IFN-γ产生的抑制作用也由所有类型的APC介导。最有效的反应是由单核细胞衍生的DC诱导的，因此构成了一个敏感的筛选模型。
The intestinal microbiota is essential for homeostasis of the local and systemic immune system, and particularly strains of lactic acid bacteria and Escherichia coli have been shown to have balancing effects on inflammatory conditions such as allergy and inflammatory bowel disease. However, in vitro assessment of the immunomodulatory effects of distinct strains may depend strongly on the cell type used as a model. To select the most appropriate model for screening of beneficial bacteria in human cells, the response to strains of intestinal bacteria of three types of antigen-presenting cells (APC) was compared; blood myeloid dendritic cells (DC), monocyte-derived DC and monocytes, and the effector response of natural killer cells and naive T cells was characterized. Maturation induced by gut-derived bacteria differed between APC, with blood DC and monocytes responding with the production of IL-6 and tumour necrosis factor-alpha to bacteria, which elicited mainly IL-10 in monocyte-derived DC. In contrast, comparable IFN-gamma production patterns were found in both natural killer cells and T cells induced by all bacteria-matured APC. An inhibitory effect of certain strains on this IFN-gamma production was also mediated by all types of APC. The most potent responses were induced by monocyte-derived DC, which thus constitute a sensitive screening model.