Arabidopsis AINTEGUMENTA mediates salt tolerance by trans-repressing SCABP8
Arabidopsis AINTEGUMENTA mediates salt tolerance by trans-repressing SCABP8
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DOI：
10.1242/jcs.172072
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发表时间：
2015-08-01
影响因子：
4
通讯作者：
Liu, Aizhong
中科院分区：
文献类型：
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作者：
Meng, Lai-Sheng;Wang, Yi-Bo;Liu, Aizhong
拟南芥AINTEGUMENTA（ANT）基因编码APETALA 2（AP 2）样转录因子，在整个茎段发育过程中控制植物器官细胞数量和器官大小。因此，ANT是植物枝条发育的关键因素。在这里，我们发现ANT在拟南芥的耐盐性中起着至关重要的作用。在35 S启动子下表达ANT的转基因植株（35 S：ANT）在高盐胁迫下表现出更敏感的表型。进一步分析表明，ANT主要在盐胁迫下的地上部响应中发挥作用。靶基因分析显示，ANT与编码推定的Ca 2+传感器的SOS 3样钙结合蛋白8（SCABP 8）的启动子结合，从而抑制SCABP 8（也称为CBL 10）的表达。据报道，scabp 8的盐敏感性在地上部组织中更为突出。遗传实验表明，SCABP 8的突变抑制了ant-knockout耐盐表型，这意味着ANT作为SCABP 8在盐胁迫下的负转录调控因子。综上所述，上述结果表明ANT是一种新的盐胁迫调节剂，ANT与SCABP 8启动子结合，介导盐耐受性。
The Arabidopsis AINTEGUMENTA (ANT) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant shoots. Here, we have found that ANT plays an essential role in conferring salt tolerance in Arabidopsis. ant-knockout mutants presented a salt-tolerant phenotype, whereas transgenic plants expressing ANT under the 35S promoter (35S:ANT) exhibited more sensitive phenotypes under high salt stress. Further analysis indicated that ANT functions mainly in the shoot response to salt toxicity. Target gene analysis revealed that ANT bound to the promoter of SOS3-LIKE CALCIUM BINDING PROTEIN 8 (SCABP8), which encodes a putative Ca2+ sensor, thereby inhibiting expression of SCABP8 (also known as CBL10). It has been reported that the salt sensitivity of scabp8 is more prominent in shoot tissues. Genetic experiments indicated that the mutation of SCABP8 suppresses the ant-knockout salt-tolerant phenotype, implying that ANT functions as a negative transcriptional regulator of SCABP8 upon salt stress. Taken together, the above results reveal that ANT is a novel regulator of salt stress and that ANT binds to the SCABP8 promoter, mediating salt tolerance.