Mapping early transcripts of herpes simplex virus type 1 by electron microscopy
Mapping early transcripts of herpes simplex virus type 1 by electron microscopy
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通过电子显微镜绘制 1 型单纯疱疹病毒的早期转录本
DOI：
10.1128/jvi.27.1.56-73.1978
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发表时间：
1978
影响因子：
5.4
通讯作者：
E. Wagner
中科院分区：
文献类型：
--
作者：
J. Stringer;L. Holland;E. Wagner
在完整的单纯疱疹病毒DNA和单纯疱疹病毒DNA限制性内切酶片段中的RNA置换循环模式表明，感染后早期与多聚核糖体相关的病毒RNA是从基因组的三个主要区域转录而来的。早期转录的一个区域在病毒DNA的短片段中，大致由结合这一片段的反向重复序列描绘。早期mRNA转录的另外两个区域位于长片段。早期mRNA转录的三个主要区域中的每一个都可以进一步分解为几个频率循环的区域，其中RNA置换环是罕见的区域。这些区域的大小从约1.5千碱基到约9千碱基不等，平均大小约为3.5千碱基。虽然这些数据不能准确地指定单个早期基因的位置，但即使在最低分辨率的情况下，也可以看到早期基因并不是完全连续的，而是沿着1型单纯疱疹病毒基因组的长度分布的。
RNA displacement loop patterns in intact herpes simplex virus DNA and herpes simplex virus DNA restriction fragments indicate that viral RNA associated with polyribosomes early after infection is transcribed from three major areas of the genome. One area of early transcription is in the short segment of the viral DNA and is roughly delineated by the inverted repeat sequences bounding this segment. The other two areas of early mRNA transcription map in the long segment. Each of three major areas of early mRNA transcription can be further resolved into several regions of freqent looping bordered by regions in which RNA displacement loops are rare. These regions range in size from about 1.5 kilobases to about 9 kilobases with a mean size of about 3.5 kilobases. Although the data do not allow precise assignment of individual early gene locations, it is seen, even at the lowest level of resolution, that the early genes are not completely contiguous but are distributed along the length of the herpes simplex type 1 viral genome.