A cGMP-dependent protein kinase gene, foraging, modifies habituation-like response decrement of the giant fiber escape circuit in Drosophila
A cGMP-dependent protein kinase gene, foraging, modifies habituation-like response decrement of the giant fiber escape circuit in Drosophila
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DOI：
10.1101/lm.31600
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发表时间：
2000-09-01
影响因子：
2
通讯作者：
Wu, CF
中科院分区：
文献类型：
--
作者：
Engel, JE;Xie, XJ;Wu, CF
果蝇巨纤维跳跃和飞行逃避反应是一个模型的生理和可塑性的感觉运动行为通路的遗传分析。我们以前建立了电诱导的巨纤维反应在完整的拴系苍蝇作为一个模型的习惯化，一种形式的非联想学习。在这里，我们表明，在习惯化协议中，该神经通路的刺激依赖性反应衰减率与PKG（cGMP依赖性蛋白激酶）活性和觅食行为相关。我们分析了自然和突变的漫游者和保姆等位基因的觅食（为）基因编码的果蝇PKG的反应递减。漫游幼虫和成人，具有较高的PKG活动，旅行显着更远，而觅食比坐在较低的PKG活动。反应递减是最迅速的基因型，以前被证明有低PKG活动和坐样觅食行为。我们还发现了自发恢复（从刺激休息期间的反应递减的逆转）和disabituation样现象（由一个新的刺激引起的反应递减的逆转）的差异。在一个完整的动物制备的电生理研究提供了第一个直接的证据，PKG可以影响可塑性在一个简单的学习范例。它增加了我们的理解复杂的相互作用的因素，可以调节巨纤维逃逸反应的敏感性，它定义了一个新的成年阶段的觅食位点的表型。最后，这些结果表明，行为相关的神经可塑性在一个确定的电路可以影响存在于自然种群的果蝇的单基因座遗传多态性。
The Drosophila giant fiber jump-and-flight escape response is a model for genetic analysis of both the physiology and the plasticity of a sensorimotor behavioral pathway. We previously established the electrically induced giant fiber response in intact tethered flies as a model for habituation, a form of nonassociative learning. Here, we show that the rate of stimulus-dependent response decrement of this neural pathway in a habituation protocol is correlated with PKG (cGMP-Dependent Protein Kinase) activity and foraging behavior. We assayed response decrement for natural and mutant rover and sitter alleles of the foraging (for) gene that encodes a Drosophila PKG. Rover larvae and adults, which have higher PKG activities, travel significantly farther while foraging than sitters with lower PKG activities. Response decrement was most rapid in genotypes previously shown to have low PKG activities and sitter-like foraging behavior. We also found differences in spontaneous recovery (the reversal of response decrement during a rest from stimulation) and a dishabituation-like phenomenon (the reversal of response decrement evoked by a novel stimulus). This electrophysiological study in an intact animal preparation provides one of the first direct demonstrations that PKG can affect plasticity in a simple learning paradigm. It increases our understanding of the complex interplay of factors that can modulate the sensitivity of the giant fiber escape response, and it defines a new adult-stage phenotype of the foraging locus. Finally, these results show that behaviorally relevant neural plasticity in an identified circuit can be influenced by a single-locus genetic polymorphism existing in a natural population of Drosophila.