Three new isoflavones from the Pueraria montana var. lobata (Willd.) and their bioactivities
Three new isoflavones from the Pueraria montana var. lobata (Willd.) and their bioactivities
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DOI：
10.1080/14786419.2017.1385008
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发表时间：
2018-12-02
影响因子：
2.2
通讯作者：
Jiang, Lihong
中科院分区：
文献类型：
--
作者：
Cui, Tinghui;Tang, Shiyun;Jiang, Lihong
从野葛中分离得到3个新的异黄酮类化合物：7-乙酰基-4‘，6-二甲氧基-异黄酮(1)、7-乙酰基-4’-羟基-6-甲氧基-异黄酮(2)和7-乙酰基-6，8-二甲氧基-4‘-羟基-异黄酮(3)，以及5个已知的黄酮类化合物(4-8)。Lobata(Wild.)通过波谱方法，包括广泛的一维和二维核磁共振技术，确定了它们的结构。对化合物1-8进行了抗烟草花叶病毒(TMV)活性评价。结果表明，化合物1和2具有较高的抗烟草花叶病毒活性，抑制率分别为36.8%和33.6%。抑制率均高于阳性对照。其他化合物也具有潜在的抗烟草花叶病毒活性，抑制率分别为21.8%~28.4%。并测定了化合物1-3对NB4、A549、SHSY5Y、PC3和MCF7等5种人肿瘤细胞株的细胞毒作用。结果表明，化合物1-3对某些人肿瘤细胞株有较弱的抑制作用，IC50在1.2-3.6mM范围内。
Three new isoflavones, 7-acetyl-4', 6-dimethoxy-isoflavone (1), 7-acetyl4'- hydroxy-6-methoxy-isoflavone (2) and 7-acetyl-6,8-dimethoxy-4'hydroxy- isoflavone (3), together with five known flavones (4-8), were isolated from the Pueraria montana var. lobata (Willd.). Their structures were elucidated by spectroscopic methods, including extensive 1Dand 2D NMR techniques. Compounds 1-8 were evaluated for their anti-tobacco mosaic virus (anti-TMV) activities. The results showed that compounds 1 and 2 exhibited high anti-TMV activities with inhibition rates of 36.8 and 33.6%, respectively. The inhibition rates are higher than that of positive control. The other compounds also showed potential anti-TMV activities with inhibition rates in the range of 21.8 similar to 28.4%, respectively. The cytotoxicities of compounds 1-3 against five human tumour cell lines (NB4, A549, SHSY5Y, PC3 and MCF7) were also tested. The results revealed that compounds 1-3 showed weak inhibitory activities against some tested human tumour cell lines with IC 50 values in the range of 1.2-3.6 mu M.[GRAPHICS].