Limited surveillance of fumonisins in brown rice and wheat harvested in Japan.
Limited surveillance of fumonisins in brown rice and wheat harvested in Japan.
复制标题
对日本收获的糙米和小麦中伏马菌素的监测有限。
DOI：
10.4315/0362-028x-72.6.1327
复制
发表时间：
2009
影响因子：
2
通讯作者：
H. Nagashima
中科院分区：
文献类型：
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作者：
M. Kushiro;Yazhi Zheng;R. Nagata;H. Nakagawa;H. Nagashima
伏马菌素是一种主要由轮枝镰刀菌（Fusarium verticillioides）产生的真菌毒素，是玉米的主要污染物。然而，在世界范围内，有零星的报道小麦中伏马菌素污染。水稻附着性真菌Gibberella fujikuroi在分类学上与F.轮枝状因此，伏马菌素污染水稻和小麦的潜在风险是显着的。在此之前，利用液相色谱-串联电喷雾质谱（LC-ESI-MS-MS）的检测方法，用于糙米中伏马菌素的检测。本研究采用LC-ESI-MS-MS技术对日本产糙米和小麦中伏马菌素的检出率进行了研究。对48个稻米样品和47个小麦样品进行了伏马菌素B型（fumonisin B1，FB 1）和伏马菌素B型（fumonisin B2，FB 2）的筛选和分析。将约1公斤水稻或小麦种子分成三个子样本，每个子样本取10克用于分析。检测限分别为0.012和0.011毫克/千克的FBt和FB 2，分别在大米样品和0.010和0.008毫克/千克的FB 1和FB 2，分别在小麦样品。以0.50 mg/kg添加到无毒大米和小麦样品中，平均回收率分别为77.6（4.2）%和84.5（3.1）%。其中一个小麦样品对FBt呈阳性，其值大于检测限，但在任何大米样品中均未发现伏马菌素。这是首次在日本小麦中检测到伏马菌素。
Fumonisins are mycotoxins mainly produced by Fusarium verticillioides, which is a major contaminant of corn. However, there are sporadic reports of fumonisin contamination in wheat worldwide. The rice adherent fungus Gibberella fujikuroi is taxonomically closely related to F. verticillioides. Therefore, the potential risk of fumonisin contamination in rice and wheat is significant. Previously, a sensitive detection method utilizing liquid chromatography with tandem electrospray mass spectrometry (LC-ESI-MS-MS) was developed for the determination of fumonisins in brown rice. In the present study, the incidence of fumonisins in brown rice and wheat harvested in Japan was investigated using LC-ESI-MS-MS. Forty-eight rice samples and 47 wheat samples were screened and analyzed for the major B-type fumonisins: fumonisin B1 (FB1) and fumonisin B2 (FB2). About 1 kg of rice or wheat seed was divided into three subsamples, and 10 g from each subsample was used for the analysis. The limits of detection were 0.012 and 0.011 mg/kg for FBt and FB2, respectively, in rice samples and 0.010 and 0.008 mg/kg for FB1 and FB2, respectively, in wheat samples. The mean (standard deviation) recoveries of FB1 spiked at 0.50 mg/kg into toxin-free rice and wheat samples were 77.6 (4.2)% and 84.5 (3.1)%, respectively. One of the wheat samples was positive for FBt with a value greater than the limit of detection,but no fumonisin was found in any of the rice samples. This is the first report of fumonisins detected in Japanese wheat.
LC-MS/MS同时分析葡萄球菌肠毒素的研究
DOI：
--
发表时间：
2018
期刊：
影响因子：
--
作者：
吉光 真人;余野木 伸哉;川津 健太郎;角谷 直哉;梶村 計志
通讯作者：
梶村 計志