Coexpression of keratinocyte growth factor and its receptor in normal and prostate cancer tissues: Possible formation of autonomous andromedin loop
Coexpression of keratinocyte growth factor and its receptor in normal and prostate cancer tissues: Possible formation of autonomous andromedin loop
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DOI：
10.1267/ahc.37.379
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Koji, T
中科院分区：
文献类型：
--
作者：
Aoki, D;Yamamoto-Fukuda, T;Koji, T
角质形成细胞生长因子(KGF)是一种雄激素依赖的上皮细胞有丝分裂原，其受体(KGFR)参与了前列腺组织细胞生长和分化的调节。本研究旨在确定KGF和KGFR在正常组织和前列腺癌组织中的表达和作用，特别是与细胞动力学有关。对41例前列腺癌石蜡包埋标本，采用免疫组织化学新提出的抗体和原位杂交法，分别从蛋白和mRNA水平分析KGF和KGFR的表达。同时检测雄激素受体(AR)的表达和Ki-67标记指数(LI)。在正常和增生性前列腺组织中，KGF mRNA和蛋白均定位于AR阳性的间质细胞，而KGFR则定位于腺上皮细胞。在前列腺癌中，14/41例KGF和KGFR同时表达，且与高Gleason评分、骨转移和Ki-67高表达密切相关。共表达KGF和KGFR的前列腺癌患者的无复发生存期明显短于其他患者。因此，我们的结果表明，KGF和KGFR在前列腺癌中的共同表达可能预示着前列腺癌的转移和增殖活动，可能是由于形成了一个自主的雄激素环。
Keratinocyte growth factor (KGF), an androgen-dependent epithelial mitogen, and its receptor (KGFR) have been implicated in the regulation of cell growth and differentiation in prostate tissue. This study was designed to determine the expression and role of KGF and KGFR in normal and prostate cancer tissues, especially in relation to cell kinetics. In 41 cases of prostate cancer in paraffin-embedded specimens, the expression of KGF and KGFR at the levels of protein and mRNA was analyzed by immuno-histochemistry using newly raised antibodies and in situ hybridization, respectively. We also examined expression of androgen receptor (AR) and Ki-67 labeling index (LI). In normal and hyperplastic prostate tissues, both KGF mRNA and protein were localized in AR positive stromal cells, while those of KGFR were localized in glandular epithelial cells. In prostate cancer, however, coexpression of KGF and KGFR was observed in 14/41 cases, and significantly correlated with high Gleason scores, bone metastasis and high Ki-67 Ll. The relapse-free survival of patients suffering from prostate cancers coexpressing KGF and KGFR was significantly shorter than that of patients from the other ones. Therefore, our results indicate that coexpression of KGF and KGFR in prostate cancer may predict metastatic and proliferative activities, possibly due to the formation of an autonomous andromedin loop.