Expression of cytokines by multipotent neural progenitor cells
Expression of cytokines by multipotent neural progenitor cells
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DOI：
10.1016/s1043-4666(03)00120-0
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发表时间：
2003-05-01
期刊：
影响因子：
3.8
通讯作者：
Berman, MA
中科院分区：
文献类型：
--
作者：
Klassen, HJ;Imfeld, KL;Berman, MA
最近对哺乳动物神经干细胞的研究强调了细胞因子信号在这些多能细胞增殖和分化中的作用。虽然已经在体内和体外证明了神经祖细胞对外源性应用的生长因子的反应性，但是很少注意到这些细胞产生细胞因子。在这里，我们使用免疫细胞化学，RT-PCR和ELISA显示，在标准生长条件下，多能神经祖细胞从人类表达多种细胞因子，包括IL-1 α，IL-1 β，IL-6，TGF-β 1，TGF-β 2，TNF-α，但不IL-2，IL-4，或IFN-γ。大鼠和小鼠的神经前体细胞在相似的条件下表达这些细胞因子中的一些，但不是全部。虽然神经祖细胞表达细胞因子的功能仍有待阐明，但已知这些信号分子参与神经发育，并可能在各种病理过程激活静止干细胞中发挥作用。(C)2003爱思唯尔科技有限公司版权所有。
Recent work with mammalian neural stem cells has highlighted the role of cytokine signaling in the proliferation and differentiation of these multipotent cells. While the responsiveness of neural progenitors to exogenously applied growth factors has been demonstrated in vivo as well as in vitro, little attention has been given to the production of cytokines by these cells. Here we use immunocytochemistry, RT-PCR, and ELISA to show that under standard growth conditions multipotent neural progenitor cells from humans express multiple cytokines including IL-1alpha, IL-1beta, IL-6, TGF-beta1, TGF-beta2, TNF-alpha, but not IL-2, IL-4, or IFN-gamma. Neural progenitor cells from rat and mouse express some, but not all, of these cytokines under similar conditions. While the function of cytokine expression by neural progenitor cells remains to be elucidated, these signaling molecules are known to be involved in neural development and may play a role in the activation of quiescent stem cells by a variety of pathological processes. (C) 2003 Elsevier Science Ltd. All rights reserved.