Dephosphorylated NSSR1 is induced by androgen in mouse epididymis and phosphorylated NSSR1 is increased during sperm maturation.
Dephosphorylated NSSR1 is induced by androgen in mouse epididymis and phosphorylated NSSR1 is increased during sperm maturation.
复制标题
雄激素在小鼠附睾中诱导去磷酸化 NSSR1，磷酸化 NSSR1 在精子成熟过程中增加
DOI：
10.1371/journal.pone.0025667
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen XH
中科院分区：
文献类型：
--
作者：
Xiao PJ;Peng ZY;Huang L;Li Y;Chen XH
NSSR1（富含神经突出丝氨酸/精氨酸的蛋白 1，或者 SRp38）是一种新发现的 RNA 剪接因子，主要在神经组织中表达。在这里，通过蛋白质印迹分析和免疫荧光染色，我们发现去磷酸化的NSSR1的表达在附睾头发育过程中显着增加。在成年小鼠中，磷酸化的NSSR1主要在上皮细胞的顶端表达，而去磷酸化的NSSR1在附睾头中以睾酮依赖性方式上调。此外，NSSR1的亚细胞免疫反应分布在附睾的不同区域也不同。对于精子，在尾部中段和顶体中检测到磷酸化的 NSSR1。此外，NSSR1在获能和顶体反应期间从精子头部释放。这些发现首次为 NSSR1 在精子成熟和受精中的潜在作用提供了证据。
NSSR1 (Neural salient serine/arginine rich protein 1, alternatively SRp38) is a newly identified RNA splicing factor and predominantly expressed in neural tissues. Here, by Western blot analysis and immunofluorescent staining, we showed that the expression of dephosphorylated NSSR1 increased significantly during development of the caput epididymis. In adult mice, phosphorylated NSSR1 was mainly expressed in the apical side of epithelial cells, and dephosphorylated NSSR1 in caput epididymis was upregulated in a testosterone dependent manner. In addition, subcellular immunoreactive distribution of NSSR1 varied in different regions of the epididymis. With respect to the sperm, phosphorylated NSSR1 was detected in the mid-piece of the tail as well as the acrosome. Furthermore, NSSR1 was released from the sperm head during the capacitation and acrosome reaction. These findings for the first time provide the evidence for the potential roles of NSSR1 in sperm maturation and fertilization.