Spatial-domain low-coherence quantitative phase microscopy for cancer diagnosis
Spatial-domain low-coherence quantitative phase microscopy for cancer diagnosis
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DOI：
10.1364/ol.35.002840
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发表时间：
2010-09-01
期刊：
影响因子：
3.6
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Wang, Pin;Bista, Rajan;Liu, Yang
提出了一种显微镜技术，即空间域低相干定量相位显微镜（SL-QPM），用于具有亚纳米灵敏度的亚细胞结构的无散斑定量相位成像。据我们所知，我们首次量化了原始未经修饰的组织学标本上细胞核的折射率。我们证明细胞核的折射率在检测癌症时高度敏感，尤其是来自癌症患者的组织学表现正常的细胞。由于该技术灵敏且不需要特殊的样品处理，因此可以传播到所有临床环境。 (C) 2010 美国光学学会
A microscopy technique, spatial-domain low-coherence quantitative phase microscopy (SL-QPM) is proposed for speckle-free, quantitative phase imaging of subcellular structures with subnanometer sensitivity. We quantified, for the first time to our knowledge, the refractive index of the cell nuclei on original unmodified histology specimens. We demonstrate that the refractive index of cell nucleus is highly sensitive in detecting cancer, especially in histologically normal-appearing cells from cancer patients. Because this technique is sensitive and does not require special sample processing, it can be disseminated to all clinical settings. (C) 2010 Optical Society of America