Mating and blood-feeding induce transcriptome changes in the spermathecae of the yellow fever mosquito Aedes aegypti
Mating and blood-feeding induce transcriptome changes in the spermathecae of the yellow fever mosquito Aedes aegypti
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DOI：
10.1038/s41598-020-71904-z
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发表时间：
2020-09-10
影响因子：
4.6
通讯作者：
Avila, Frank W.
中科院分区：
文献类型：
--
作者：
Camargo, Carolina;Ahmed-Braimah, Yasir H.;Avila, Frank W.
埃及伊蚊是影响人类健康的众多病毒的主要媒介。由于已提出通过操纵繁殖来抑制蚊子数量，因此阐明使雄性和雌性成功繁殖的生物学过程是必要的。一个重要的过程是将雌性精子储存在称为精囊的特殊结构中。埃及伊蚊雌性通常只交配一次，这要求它们在一生中保持精子的活力，使它们产下的卵子受精。精子囊基因产物是果蝇精子储存和精子存活所必需的，而冈比亚按蚊则需要精子囊衍生的血红素过氧化物酶。Ae的产物。埃及伊蚊的精子科及其对交配的反应在很大程度上是未知的。此外，尽管雌蚊吸血对非自体蚊子繁殖至关重要，但在任何生殖组织中，对食血的转录反应仍未明确。我们在交配后6、24和72小时对未喂食的雌性、只交配的雌性和交配后喂养的雌性的精子进行了RNAseq分析，并在每个时间点发现了各组中显著差异表达的基因。交配后的血餐在精囊中诱导了比单独交配更大的转录反应。这项研究提供了第一个观点，在精子被诱导的mRNA变化的血餐在交配的雌性。
Aedes aegypti mosquitoes are the primary vectors of numerous viruses that impact human health. As manipulation of reproduction has been proposed to suppress mosquito populations, elucidation of biological processes that enable males and females to successfully reproduce is necessary. One essential process is female sperm storage in specialized structures called spermathecae. Aedes aegypti females typically mate once, requiring them to maintain sperm viably to fertilize eggs they lay over their lifetime. Spermathecal gene products are required for Drosophila sperm storage and sperm viability, and a spermathecal-derived heme peroxidase is required for long-term Anopheles gambiae fertility. Products of the Ae. aegypti spermathecae, and their response to mating, are largely unknown. Further, although female blood-feeding is essential for anautogenous mosquito reproduction, the transcriptional response to blood-ingestion remains undefined in any reproductive tissue. We conducted an RNAseq analysis of spermathecae from unfed virgins, mated only, and mated and blood-fed females at 6, 24, and 72 h post-mating and identified significant differentially expressed genes in each group at each timepoint. A blood-meal following mating induced a greater transcriptional response in the spermathecae than mating alone. This study provides the first view of elicited mRNA changes in the spermathecae by a blood-meal in mated females.