Translocation of epidermal growth factor to the hepatocyte nucleus during rat liver regeneration.
Translocation of epidermal growth factor to the hepatocyte nucleus during rat liver regeneration.
复制标题
大鼠肝脏再生过程中表皮生长因子易位至肝细胞核。
DOI：
10.1016/s0016-5085(87)91084-5
复制
发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Jones,AL
中科院分区：
文献类型：
--
作者：
Raper,SE;Burwen,SJ;Barker,ME;Jones,AL
为探讨外源性表皮生长因子（EGF）在再生肝中的命运，将1251 I标记的EGF在70%肝切除后不同时间注入大鼠门静脉。表皮生长因子在研究的所有时间点（0、4、8、16和36 h）均被肝残体摄取，但在肝切除术后8 h（a）大量表皮生长因子被肝脏保留，（B）胆汁中出现的表皮生长因子降解产物显著减少。注射125 I-EGF后1小时再生肝的电子显微镜放射自显影表明，27%的颗粒与肝细胞核相关联，而在假手术对照组中为0.5%。与溶酶体隔室相关的颗粒也随之减少。从暴露于125 I-EGF的再生肝脏中分离的细胞核与对照肝脏的细胞核相比，放射性也增加了三倍。近70%的核放射性可与EGF的特异性抗体沉淀，一小部分似乎是高分子量复合物的一部分。这些数据支持的假设，即在前S期的肝再生，表皮生长因子是易位到细胞核，而不是溶酶体，并可能参与启动脱氧核糖核酸合成或改变基因表达。
To determine the fate of exogenous epidermal growth factor (EGF) in regenerating liver, 1251 I-labeled EGF was injected into rat portal veins at various times after 70% hepatectomy. Epidermal growth factor was taken up by the liver remnant at all time points studied (0, 4, 8, 16, and 36 h), but at 8 h after hepatectomy (a) a large quantity was retained by the liver and (b) EGF degradation products appearing in the bile decreased markedly. Electron microscopic autoradiography of the regenerating livers 1 h after injection of 125I-EGF demonstrated that 27% of the grains were associated with hepatocyte nuclei compared to 0.5% in shamoperated controls. There was also a concomitant decrease in grains associated with the lysosomal compartment. Nuclei isolated from regenerating livers exposed to 125I-EGF also demonstrated a threefold increase in radioactivity compared to nuclei from control livers. Nearly 70% of nuclear radioactivity was precipitable with a specific antibody to EGF, and a small fraction appeared to be part of a high molecular weight complex. These data support the hypothesis that during the pre-S phase of liver regeneration, EGF is translocated to the nucleus rather than to lysosomes, and may participate in the initiation of deoxyribonucleic acid synthesis or alteration of gene expression.