SCO5351 is a pleiotropic factor that impacts secondary metabolism and morphological development in Streptomyces coelicolor
SCO5351 is a pleiotropic factor that impacts secondary metabolism and morphological development in Streptomyces coelicolor
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SCO5351 是一种影响天蓝色链霉菌次生代谢和形态发育的多效性因子。
DOI：
10.1093/femsle/fny150
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发表时间：
2018-09-01
影响因子：
2.1
通讯作者：
Pang, Xiuhua
中科院分区：
文献类型：
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作者：
Lu, Ting;Zhu, Yanping;Pang, Xiuhua
天蓝色链霉菌的基因组编码数百个推定的调控蛋白，其中大多数是未知的功能，包括SCO 5351。在这项研究中，我们确定sco 5351的缺失在很大程度上消除了放线菌紫素（ACT）的生产，并减少了钙依赖性抗生素（CDA）的生产。全面的转录分析表明，转录的ACT途径的基因，包括途径特异性调节actII-orf 4和那些参与建设的化合物，是显着较低的三角洲sco 5351在生长后期。然而，CDA簇中基因的转录仅在生长早期在Delta sco 5351中显著降低，表明SCO 5351在整个生长过程中具有调节作用。与Delta sco 5351的观察结果相似，ACT的产生被可能参与SCO 5351二聚化的三个保守氨基酸的诱变所阻断，表明蛋白质二聚化对SCO 5351的功能至关重要。此外，sco 5351的破坏延迟了气生菌丝体和孢子在测试条件下的形成，并且与此一致，与孢子形成相关的发育基因的转录在Delta sco 5351中减少，这意味着sco 5351参与发育控制。我们的研究结果表明，SCO 5351作为一个多效性的调节剂，在次级代谢和形态发育的S。天蓝色。
The genome of Streptomyces coelicolor encodes hundreds of putative regulatory proteins, most of which are of unknown function, including SCO5351. In this study, we determined that deletion of sco5351 largely abrogates production of actinorhodin (ACT) and reduces production of the calcium-dependent antibiotic (CDA). Comprehensive transcriptional analyses indicated that transcription of genes of the ACT pathway, including the pathway-specific regulator actII-orf4 and those involved in the building of the chemical compound, was markedly lower in Delta sco5351 in the late growth phase. However, transcription of genes in the CDA cluster was notably reduced in Delta sco5351 only in the early growth phase, suggesting that SCO5351 has a regulatory role throughout growth. Similar to the observations with Delta sco5351, ACT production was blocked by mutagenesis of three conserved amino acids potentially involved in dimerization of SCO5351, indicating that protein dimerization is critical to the function of SCO5351. In addition, disruption of sco5351 delayed the formation of aerial mycelium and spores under the conditions tested and, consistent with this, transcription of developmental genes associated with spore formation was reduced in Delta sco5351, implying that SCO5351 is involved in developmental control. Our findings reveal SCO5351 as a pleiotropic regulator with roles in both secondary metabolism and morphological development in S. coelicolor.