Effect of acetylsalicylic acid on total myenteric neurons in mice experimentally infected with Trypanosoma cruzi
Effect of acetylsalicylic acid on total myenteric neurons in mice experimentally infected with Trypanosoma cruzi
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DOI：
10.1590/0001-3765201920180389
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
MOREIRA, NEIDE M.
中科院分区：
文献类型：
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作者：
SOUZA, NOEMI D. DE;BELIN, BRUNA S.;MOREIRA, NEIDE M.
我们研究了乙酰水杨酸 (ASA) 对克氏锥虫感染小鼠降结肠肌间神经元总数的影响。 60日龄雄性Swiss小鼠35只，分为对照组（C组）、ASA处理对照组（CA组）、感染组（I组）、ASA处理感染组（IA组）。 IA组和I组腹腔内接种克氏锥虫Y株锥鞭毛体共计1300只。 CA组和IA组腹腔注射ASA。感染后 75 天 (dpi)，处死所有动物。结肠中的神经元用吉姆萨染色、定量和测量。从寄生虫血症曲线可以看出，IA 组和 I 组之间没有观察到感染过程的差异。与C和I组相比，CA和IA组的乙酰水杨酸治疗没有改变肌间神经元的总数。与C和I组相比，CA和IA组的核面积、细胞质面积和神经元体面积增加。未来的研究应阐明 ASA 针对慢性期恰加斯病的作用机制。
We investigated the effects of acetylsalicylic acid (ASA) on the total myenteric neuronal population in the descending colon in Trypanosoma cruzi-infected mice. Thirty-five male Swiss mice, 60 days old, were divided into a control group (C group), control group treated with ASA (CA group), infected group (I group), and infected group treated with ASA (IA group). A total of 1300 trypomastigotes of the Y strain of T. cruzi were intraperitoneally inoculated in the IA and I groups. The CA and IA groups were treated with ASA intraperitoneally. At 75 days post-infection (dpi), all of the animals were sacrificed. Neurons in the colon were stained with Giemsa, quantified, and measured. No difference in the course of infection was observed between the IA and I groups, reflected by the parasitemia curve. Acetylsalicylic acid treatment in the CA and IA groups did not alter the total number of myenteric neurons compared with the C and I groups. The CA and IA groups exhibited an increase in the nuclear area, cytoplasmic area, and neuronal body area compared with the C and I groups. Future studies should elucidate the mechanism of action of ASA against Chagas' disease in the chronic phase.