Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage.
Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage.
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DOI：
10.1002/jsfa.5937
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发表时间：
2013-05
影响因子：
4.1
通讯作者：
T. Zhao;Ye Zhou;G. Mao;Y. Zou;Jiang-li Zhao;S. Bai;Liuqing Yang;Xiangyang Wu
中科院分区：
文献类型：
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作者：
T. Zhao;Ye Zhou;G. Mao;Y. Zou;Jiang-li Zhao;S. Bai;Liuqing Yang;Xiangyang Wu
研究背景中华鲟（Acipenser sinensis）是我国重要的渔业资源，是我国重要的珍稀鱼类。为了保护这一物种免受过度捕捞、筑坝和污染，近年来人工繁殖得到迅速发展。然而，来自中华鲟的软骨含有丰富的硫酸软骨素（CS），目前在食用鱼之后被作为固体废物丢弃。研究中华鲟软骨壳聚糖的提取、纯化及性质。结果最佳提取工艺条件为：NaOH与软骨粉的质量比为9.2，NaOH浓度为4.4%，提取时间为3.9h。在此优化条件下，中华鲟软骨壳聚糖的粗品得率为26.51%，与模型预测值（26.54%）基本一致。通过DEAE-52纤维素和Sephadex G-100柱层析纯化，得到单一级分CS-11。其表征表明CS-11与多糖骨架结构-4GlcAβ-3GalNβ-一致，并且以软骨素-4-硫酸盐和软骨素-6-硫酸盐的形式存在。结论本研究结果为促进中华鲟资源的开发利用提供了依据，对中华鲟软骨CS在食品工业中的开发利用具有重要意义。
BACKGROUND Chinese sturgeon (Acipenser sinensis), a rare species, is an important fishery resource in China. To save this species from overfishing, damming and pollution, artificial propagation has been developed rapidly in recent years. However, the cartilage from Chinese sturgeon, which contains an abundance of chondroitin sulfate (CS), is currently discarded as solid waste after consumption of the fish. The aim of this study was to investigate the extraction, purification and characterisation of CS from Chinese sturgeon cartilage. RESULTS The optimal extraction parameters were a ratio of aqueous NaOH to cartilage powder of 9.2, a concentration of aqueous NaOH of 4.4% and an extraction time of 3.9 h. Under these optimal conditions the yield of crude CS from Chinese sturgeon cartilage was 26.51%, in agreement with the model prediction (26.54%). Purification by DEAE-52 cellulose and Sephadex G-100 column chromatography yielded a single fraction, CS-11. Its characterisation indicated that CS-11 was consistent with the polysaccharide backbone structure -4GlcAβ-3GalNβ- and was present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate. CONCLUSION The results of this study provide a basis for promoting the utilisation of Chinese sturgeon resources and are significant for the development and utilisation of CS from Chinese sturgeon cartilage in the food industry.