Assessment of aflatoxin exposure using serum and urinary biomarkers in Sao Paulo, Brazil: A pilot study
Assessment of aflatoxin exposure using serum and urinary biomarkers in Sao Paulo, Brazil: A pilot study
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DOI：
10.1016/j.ijheh.2015.12.003
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发表时间：
2016-05-01
影响因子：
6
通讯作者：
Oliveira, Carlos A. F.
中科院分区：
文献类型：
--
作者：
Jager, Alessandra V.;Tonin, Fernando G.;Oliveira, Carlos A. F.
本研究的目的是通过测定血清黄曲霉毒素B（1）-赖氨酸和尿黄曲霉毒素生物标志物（AFM（1）和AF B（1）-N-7-鸟嘌呤），评价来自巴西皮拉苏农加的个体对膳食黄曲霉毒素B-1（AF B（1））和M-1（AFM（1））的人体暴露。参与者是从圣保罗大学校园的员工中招募的，该大学从2011年6月至2012年3月每三个月提供一次来自他们家中的食物样本以及血清和尿液样本。黄曲霉毒素的每日可能摄入量（PDI）是通过使用样本收集时收集的食品分析结果和24小时饮食回忆问卷的数据来估计的。采用高效液相色谱-荧光检测法对食品样品中的黄曲霉毒素B（1）和原子力显微镜（1）进行分析。通过串联质谱法测定血清和尿液中的生物标志物。在38份谷类食品和31份乳制品样品中分别检测出AFB（1）和AFM（1），检出率分别为28%（N = 136）和36%（N = 86）。AFB（1）-赖氨酸和AFB（1）-N-7-鸟嘌呤在血清或尿液样品中分别未检出。然而，AFM（1）在74个尿液样本（65%）中发现，在4个采样时间的平均水平范围为0.37 +/- 0.23至1.70 +/- 2.88 pg/mg肌酐。不同采样时间的平均PDI不同，范围为0.09 +/- 0.09至1.35 +/- 5.98 ng/kg体重/天。在巴西首次发现尿液中AFM（1）浓度与采样1（2011年6月）中总黄曲霉毒素（AFB（1）+ AFM（1））的PDI之间存在适度但显著的相关性（r = 0.45; p = 0.03; N = 23）。尿AEA被证实是非常敏感的监测人体暴露于膳食黄曲霉毒素。需要使用血清和尿液生物标志物进行进一步研究，以估计巴西高风险地区人群的黄曲霉毒素暴露。(C)2015 Elsevier GmbH. All rights reserved.
The aim of this study was to evaluate the human exposure of individuals from Pirassununga, Brazil, to dietary aflatoxins B-1 (AFB(1)) and M-1 (AFM(1)) by determination of serum AFB(1)-lysine and urinary aflatoxin biomarkers (AFM(1) and AFB(1)-N-7-guanine). The participants were recruited among employees from a Campus of the University of Sao Paulo, which provided food samples from their homes, as well as serum and urine samples four times every three months, from June 2011 until March 2012. The probable daily intake (PDI) of aflatoxin was estimated by using the results from analysis of food products collected by the time of samples collection, and data from a 24-hour dietary recall questionnaire. Analyses of AFB(1) and AFM(1) in food samples were conducted by high-performance liquid chromatography with fluorescence detection. Biomarkers in serum and urine were determined by tandem mass spectrometry. AFB(1) and AFM(1) were detected in 38 samples of cereals (28%, N = 136) and 31 milk products (36%, N = 86), respectively. AFB(1)-lysine and AFB(1)-N-7-guanine and were not detected in serum or urine samples, respectively. However, AFM(1) was found in 74 urine samples (65%), at mean levels in the 4 sampling times ranging from 0.37 +/- 0.23 to 1.70 +/- 2.88 pg/mg creatinine. The mean PDI varied among different sampling times, ranging from 0.09 +/- 0.09 to 1.35 +/- 5.98 ng/kg body weight/day. A modest though significant correlation (r = 0.45; p = 0.03; N = 23) was found for the first time in Brazil between the AFM(1) concentration in urine and the PDI for total aflatoxins (AFB(1) + AFM(1)) in sampling 1 (June 2011). Urinary AEA was confirmed as very sensitive for monitoring the human exposure to dietary aflatoxin. Further studies using serum and urinary biomarkers are needed to estimate the aflatoxin exposure of populations in higher risk areas in Brazil. (C) 2015 Elsevier GmbH. All rights reserved.