Isolation and characterization of Staphylococcus argenteus strains from retail foods and slaughterhouses in Japan
Isolation and characterization of Staphylococcus argenteus strains from retail foods and slaughterhouses in Japan
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DOI：
10.1016/j.ijfoodmicro.2021.109503
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发表时间：
2022-02-16
影响因子：
5.4
通讯作者：
Miyake, Masami
中科院分区：
文献类型：
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作者：
Wakabayashi, Yuki;Takemoto, Kohei;Miyake, Masami
金黄色葡萄球菌复合群中的一个新菌种是银色葡萄球菌。已知它会引起各种人类疾病，如皮肤和软组织感染、败血症和葡萄球菌食物中毒，尽管感染源尚未明确描述。在食物中毒个案中，食物中的细菌污染来源不明。本研究检查了S.在日本的零售新鲜食品和家禽屠宰场。在642份食品样品中，成功分离出沙门氏菌。在151份鸡样品中，21份（13.9%）获得了银染。在零售市场的猪肉、牛肉、鱼或蔬菜中没有分离得到证实。多位点序列分型结果显示，21株分离物可分为4个序列型（ST），而ST又可分为14个亚型。所有的食物分离物都对甲氧西林敏感，并且没有显示出Panton-Valentine杀白细胞素基因的阳性。从同一地区的两个家禽屠宰场分离到14株S.仅从一个屠宰场成功地分离出银色菌株。14株菌中有13株是在一定时间内从家禽屠宰场和屠宰场环境中分离到的，均为具有相同SPA型的ST 5961。此外，在相同的13个菌株的核心基因组上检测到的单核苷酸变异（SNV）的数量在0和17之间，表明S.设施内的银色菌株此外，来自鸡肉的一个分离物也与屠宰场分离物的相同谱系遗传相关，
Staphylococcus argenteus has been recently established as a novel species of Staphylococcus aureus complex. It is known to cause various human diseases, such as skin and soft-tissue infections, sepsis, and staphylococcal food poisoning, although the source of infection has not been clearly described. In food poisoning cases, the source of bacterial contamination in food is unknown. This study examined the prevalence of S. argenteus among retail fresh food and poultry slaughterhouses in Japan. Among 642 food samples examined, successful isolation of S. argenteus was achieved in 21 of 151 (13.9%) chicken samples. No isolations from pork, beef, fish, or vegetables in retail markets were confirmed. Multiple-locus sequence typing revealed that the 21 isolates were classified into four sequence types (ST) that were divided into 14 subtypes using spa-typing. All food isolates were susceptible to methicillin and did not show positivity for the Panton-Valentine leukocidin gene. When bacteria were isolated from two poultry slaughterhouses in the same region, 14 S. argenteus strains were successfully isolated from only one slaughterhouse. Thirteen of 14 strains were isolated from a poultry carcass and slaughterhouse environments during a certain sampling period and were all classified as ST5961 with identical spa-type. Also, the number of single nucleotide variants (SNVs) detected on the core genomes of the same 13 strains were between 0 and 17, suggesting a long-term inhabitation of an S. argenteus strain inside the facility. Furthermore, one isolate from chicken meat was also genetically linked with the same lineage of slaughterhouse isolates, with