Effects of dehydration and blockade of angiotensin II AT1 receptor on stress hormones and anti-oxidants in the one-humped camel
Effects of dehydration and blockade of angiotensin II AT1 receptor on stress hormones and anti-oxidants in the one-humped camel
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DOI：
10.1186/1746-6148-9-232
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发表时间：
2013-11-19
影响因子：
2.6
通讯作者：
Adem, Abdu
中科院分区：
文献类型：
--
作者：
Ali, Mahmoud Alhaj;Kazzam, Elsadig;Adem, Abdu
背景：本研究的目的是首次提供在存在或不存在血管紧张素 II (Ang II) AT1 受体阻滞剂（氯沙坦）的情况下长期脱水（20 天）后血浆儿茶酚胺、皮质醇、谷胱甘肽和丙二醛与时间匹配的非脱水对照骆驼的血浆儿茶酚胺、皮质醇、谷胱甘肽和丙二醛活性的数据，并记录对照中肝脏和肾脏匀浆中谷胱甘肽和丙二醛活性的反应，脱水洛沙坦处理和脱水骆驼。研究了 18 只雄性骆驼，其中 6 只脱水（对照组）、6 只脱水并用氯沙坦治疗（处理组）和 6 只脱水未处理（脱水）。结果：与时间匹配的对照组相比，处理组和脱水组的血浆去甲肾上腺素和多巴胺水平均显着升高（P < 0.01），而与时间匹配的对照组相比，血浆肾上腺素水平显着降低（P < 0.05）。来控制。与对照组相比，治疗组和脱水组的血浆皮质醇也显着增加（P < 0.01）。治疗组和脱水组的血浆、肝脏和肾匀浆中的谷胱甘肽水平显着增加（P < 0.05）。同样，治疗组和脱水组的血浆、肝脏和肾脏匀浆中的丙二醛水平也显着增加。结论：总而言之，本研究的结果表明，脱水显着增加了去甲肾上腺素、多巴胺和皮质醇的循环水平，但降低了血浆肾上腺素。同样，氯沙坦显示出与脱水相似的效果。此外，这项研究表明，单独脱水或与氯沙坦联合脱水会导致血浆、肝脏和肾脏匀浆中谷胱甘肽和丙二醛活性显着增加，可能是为了抵消自由基的潜在破坏作用。血管紧张素 II AT1 受体的阻断并没有显着改变这些指数中脱水的反应。
Background: The objective of this study was to provide for the first time data on plasma catecholamines, cortisol, glutathione and malondialdehyde after long term dehydration (20 days) in the presence and absence of angiotensin II (Ang II) AT1 receptor blocker (losartan) versus levels in time-matched, non-dehydrated control camels and to record the responses of glutathione and malondialdehyde activity in liver and kidney homogenates in control, dehydrated-losartan treated and dehydrated camels. Eighteen male camels were studied, six hydrated (control group), six dehydrated and treated with losartan (treated group) and six dehydrated not treated (dehydrated).Results: Plasma levels of norepinephrine and dopamine were significantly increased (P < 0.01) in both treated and dehydrated groups compared to time matched control, whereas Plasma epinephrine level showed significant decrease (P < 0.05) in both treated and dehydrated groups compared to control. Plasma cortisol also showed significant increase (P < 0.01) in both treated and dehydrated groups compared to control. Glutathione levels in plasma, liver and kidney homogenates for both treated and dehydrated groups reveled significant increase (P < 0.05) Likewise, malondialdehyde levels in plasma, liver and kidney homogenates were substantially and significantly increased in both treated and dehydrated groups.Conclusion: In conclusion, the results of this study demonstrated that dehydration substantially increased the circulating levels of norepinephrine, dopamine and cortisol but decreased plasma epinephrine. Similarly, losartan showed similar effects to that of dehydration. In addition, this investigation showed dehydration alone or in combination with losartan induced significant increments in glutathione and malondialdehyde activities in plasma, liver and kidney homogenates, presumably in order to counteract the potentially damaging effects of free radicals. Blockade of angiotensin II AT1 receptors did not alter significantly the response of dehydration in any of these indices.