Non-Ischemic Heart Failure With Reduced Ejection Fraction Is Associated With Altered Intestinal Microbiota
Non-Ischemic Heart Failure With Reduced Ejection Fraction Is Associated With Altered Intestinal Microbiota
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DOI：
10.1253/circj.cj-17-1285
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发表时间：
2018-06-01
影响因子：
3.3
通讯作者：
Sakata, Yasushi
中科院分区：
文献类型：
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作者：
Katsimichas, Themistoklis;Ohtani, Tomohito;Sakata, Yasushi
背景：研究表明，射血分数降低的心力衰竭（HFrEF）是一种全身炎症状态，可能是由微生物产物通过受损的肠道屏障进入血液而引发的。然而，HFrEF 患者的肠道菌群是否表现出菌群失调尚不清楚。 方法和结果：通过对粪便样本中提取的细菌 DNA 进行 16S rRNA 分析，对 28 名非缺血性 HFrEF 患者和 19 名健康对照进行评估。处理测序数据后，对细菌进行分类，利用多样性指数检查微生物生态，并比较组间共同核心属的相对丰度。此外，我们预测了细菌代谢途径的基因携带，并推断了多个分类水平上的微生物相互作用网络。两组的细菌群落均以厚壁菌门和拟杆菌门为主。两组中最丰富的属是拟杆菌属。尽管群体之间的多样性没有差异，但通过 beta 多样性指标进行的排序揭示了各群体在变异组成部分之间的分离。患者的共同核心微生物群中链球菌和韦荣球菌含量丰富，而 SMB53 则减少。氨基酸、碳水化合物、维生素和外源代谢的基因家族在各组之间显示出显着差异。交互网络揭示了患者体内细菌之间存在更高程度的相关性。结论：与健康受试者相比，非缺血性 HFrEF 患者的肠道微生物群落表现出多维差异。
Background: Research suggests that heart failure with reduced ejection fraction (HFrEF) is a state of systemic inflammation that may be triggered by microbial products passing into the bloodstream through a compromised intestinal barrier. However, whether the intestinal microbiota exhibits dysbiosis in HFrEF patients is largely unknown.Methods and Results: Twenty eight non-ischemic HFrEF patients and 19 healthy controls were assessed by 16S rRNA analysis of bacterial DNA extracted from stool samples. After processing of sequencing data, bacteria were taxonomically classified, diversity indices were used to examine microbial ecology, and relative abundances of common core genera were compared between groups. Furthermore, we predicted gene carriage for bacterial metabolic pathways and inferred microbial interaction networks on multiple taxonomic levels. Bacterial communities of both groups were dominated by the Firmicutes and Bacteroidetes phyla. The most abundant genus in both groups was Bacteroides. Although a diversity did not differ between groups, ordination by beta diversity metrics revealed a separation of the groups across components of variation. Streptococcus and Veillonella were enriched in the common core microbiota of patients, while SMB53 was depleted. Gene families in amino acid, carbohydrate, vitamin, and xenobiotic metabolism showed significant differences between groups. Interaction networks revealed a higher degree of correlations between bacteria in patients.Conclusions: Non-ischemic HFrEF patients exhibited multidimensional differences in intestinal microbial communities compared with healthy subjects.