Fifteen polymorphic microsatellite loci in Wiebesia pumilae (Hill) (Agaonidae)
Fifteen polymorphic microsatellite loci in Wiebesia pumilae (Hill) (Agaonidae)
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Wiebesia pumilae (Hill) (Agaonidae) 的 15 个多态性微卫星位点
DOI：
10.1007/s12686-009-9046-3
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发表时间：
2009-12-01
影响因子：
1.1
通讯作者：
Chen, Yan
中科院分区：
文献类型：
--
作者：
Liu, Min;Wang, Xiao-Yan;Chen, Yan
授粉的无花果黄蜂和它们的寄主建立了共同进化和性别比例研究的天堂。高度多态的分子标记将为此类研究提供极大的帮助。从无花果专性传粉者小蜂中开发了15个微卫星标记，并对来自浙江永嘉县的22只中国黄蜂进行了遗传多样性分析。15个座位共检测到74个等位基因，每个座位的等位基因数在2~9个之间，观察到的杂合度为0.190~0.727，期望杂合度为0.483~0.831。由于杂合子缺失，7个基因座显著偏离了Hardy-Weinberg平衡。没有一对基因座表现出显著的连锁不平衡。这些微卫星标记将为研究短小木霉的遗传结构及其与寄主短小木霉的共同进化提供有用的工具。
Pollinating fig wasps and their hosts establish a paradise for coevolution and sex ratio studies. High polymorphic molecular markers would be a great assistance for such studies. We developed 15 microsatellite markers from Wiebesia pumilae, the obligate pollinator of Ficus pumila, and estimated the polymorphism with 22 individual wasps from Yongjia County, Zhejiang of China. A total of 74 alleles were detected in the 15 loci and the alleles per locus ranged from 2 to 9. The observed (HO) and expected (HE) heterozygosities were 0.190–0.727 and 0.483–0.831, respectively. Significant deviation from Hardy–Weinberg equilibrium was found at seven loci due to heterozygote deficits. No locus pair showed significant linkage disequilibrium. These microsatellite markers will provide a useful tool for studying the genetic structure of W. pumilae, and its coevolution with the host F. pumila.