The SNF5‑type protein BUSHY regulates seed germination via the gibberellin pathway and is dependent on HUB1 in Arabidopsis
The SNF5‑type protein BUSHY regulates seed germination via the gibberellin pathway and is dependent on HUB1 in Arabidopsis
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SNF5-型蛋白 BUSHY 通过赤霉素途径调节种子萌发，并且依赖于拟南芥中的 HUB1
DOI：
10.1007/s00425-021-03767-1
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发表时间：
2022
期刊：
影响因子：
4.3
通讯作者：
Yongxiu Liu
中科院分区：
文献类型：
--
作者：
Zhi Wang;Hong Cao;Cun Zhang;Fengying Chen;Yongxiu Liu
主要结论SNF 5型蛋白BUSHY通过依赖于HUB 1的赤霉素途径参与拟南芥种子萌发的调控。一些组分在胚胎发育和种子成熟中起作用，然而，SNF 5型蛋白BUSHY（BSH）是否在与种子相关的性状中起作用目前尚不清楚。在我们的研究中，我们发现BSH的功能缺失突变导致拟南芥种子萌发增加，BSH的转录在种子中被赤霉素（GA）抑制剂多效唑（PAC）诱导，并且BSH在种子萌发过程中调节GA途径基因的表达，如赤霉素3-氧化酶1（GA 3 OX 1）、赤霉素刺激的拟南芥4（GASA 4）和GASA 6。遗传分析表明，BSH在GA 3 OX 1和GA 3 OX 2的下游部分起作用，BSHGA 3 OX 1和GA 3 OX 2三突变体的种子发芽率明显提高。此外，BSH响应PAC对种子萌发的调节依赖于HUB 1。这些结果为了解植物激素对种子萌发的调控机制提供了新的见解和线索。
Main conclusionThe SNF5-type protein BUSHY plays a role in the regulation of seed germination via the gibberellin pathway dependent on HUB1 inArabidopsis thaliana.AbstractSWITCH/SUCROSE NONFERMENTING (SWI/SNF) complexes play diverse roles in plant development. Some components have roles in embryo development and seed maturation, however, whether the SNF5-type protein BUSHY (BSH), one of the components, plays a role inArabidopsisseed related traits is presently unclear. In our study, we show that a loss-of-function mutation in BSH causes increased seed germination inArabidopsis.BSHtranscription was induced by the gibberellin (GA) inhibitor paclobutrazol (PAC) in the seed, and BSH regulates the expression of GA pathway genes, such asGibberellin 3-Oxidase 1(GA3OX1),Gibberellic Acid-Stimulated Arabidopsis 4(GASA4), andGASA6during seed germination. A genetic analysis showed that seed germination was distinctly improved in thebshga3ox1ga3ox2triple mutant, indicating that BSH acts partially downstream of GA3OX1 and GA3OX2. Moreover, the regulation of seed germination by BSH in response to PAC is dependent on HUB1. These results provide new insights and clues to understand the mechanisms of phytohormones in the regulation of seed germination.