Pyridine Nucleotide Redox State and Blood Flow of the Cerebral Cortex Following Middle Cerebral Artery Occlusion in the Cat
Pyridine Nucleotide Redox State and Blood Flow of the Cerebral Cortex Following Middle Cerebral Artery Occlusion in the Cat
复制标题
猫大脑中动脉闭塞后吡啶核苷酸氧化还原状态和大脑皮层血流
作者：
M. Ginsberg;M. Reivich;K. Harbig
急性变化的氧化还原状态的猫大脑皮层中的大脑中动脉（MCA）闭塞后进行了研究，并与局部脑血流的变化在缺血性皮质放射自显影确定。通过经眶入路完成动脉闭塞。皮质荧光和反射光信号记录从中央MCA领土的装置分束荧光计，并校正血管内血红蛋白的变化的荧光信号的衍生。动脉闭塞后，皮质NADH荧光迅速增加，在30至70秒内达到峰值，为满量程的20%至40%。随后在接下来的几分钟内荧光缓慢线性下降。皮质NADH荧光的行为是不受周围空气的皮质表面用氮气置换。动脉闭塞后2~15分钟，最缺血脑回的平均局部血流量值为对侧非缺血半球相应值的21%~23%。在个体动物中，动脉闭塞后的峰值NADH荧光值与受影响的缺血脑回中血流减少的程度相关（P <0.05）。
Acute changes in the redox state of NADH in the cerebral cortex of cats were investigated following occlusion of the middle cerebral artery (MCA) and were correlated with alterations of regional cerebral blood flow in the ischemic cortex determined autoradiographically. Arterial occlusion was accomplished via the transorbital approach. Cortical fluorescence and reflected light signals were recorded from the central MCA territory by means of a beam-splitting fluorometer, and a fluorescence signal corrected for alterations in intravascular hemoglobin was derived. Following arterial occlusion, there was a rapid increase in cortical NADH fluorescence, peaking within 30 to 70 seconds at 20% to 40% of full scale. This was followed by a slow linear decline in fluorescence over the next several minutes. The behavior of cortical NADH fluorescence was unaffected by replacement of the ambient air over the cortical surface with nitrogen. Mean regional blood flow values in the most ischemic gyri two to 15 minutes following arterial occlusion were 21% to 23% of the corresponding values in the opposite, nonischemic hemisphere. In individual animals, peak NADH fluorescence values following arterial occlusion correlated with the extent of blood flow reduction in the affected ischemic gyri (P < 0.05).