ERF108 from Poncirus trifoliata (L.) Raf. functions in cold tolerance by modulating raffinose synthesis through transcriptional regulation of PtrRafS
ERF108 from Poncirus trifoliata (L.) Raf. functions in cold tolerance by modulating raffinose synthesis through transcriptional regulation of PtrRafS
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ERF108 来自枳 (Poncirus trifoliata (L.) Raf)。
DOI：
10.1111/tpj.15465
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发表时间：
2021-09-02
期刊：
影响因子：
7.2
通讯作者：
Liu, Ji-Hong
中科院分区：
文献类型：
--
作者：
Khan, Madiha;Hu, Jianbing;Liu, Ji-Hong
乙烯响应因子（ERFs）是植物特异性转录因子，参与冷胁迫反应，棉子糖在植物中积累。然而，ERFs是否通过调节棉子糖的合成而在耐寒性中发挥作用仍然是一个谜。本研究从橙子（Poncirus trifoliata（L.）Raf.），一种耐寒植物，与柑橘属植物近缘。PtrERF 108定位于细胞核中并具有转录激活活性。PtrERF 108的过表达增强了转基因柠檬的耐冷性，而VIGS介导的三叶橙子中PtrERF 108的敲低则大大提高了转基因柠檬的冷敏感性。转录组分析表明，PtrERF 108过表达引起广泛的重编程基因与信号转导，生理过程和代谢途径。其中，棉子糖合酶（RafS）编码基因，PtrRafS，被确认为PtrERF 108的直接目标。过表达PtrERF 108的转基因植株中RafS活性和棉子糖含量显著增加，但在低温条件下VIGS植株中显著降低。同时，外源补充棉子糖可以恢复PtrERF 108沉默植株的耐冷性，而VIGS介导的PtrRafS敲低导致冷敏感表型。综上所述，目前的结果表明，PtrERF 108通过调节PtrRafS来调节棉子糖的合成，从而在耐冷性中发挥积极作用。我们的研究结果揭示了一个新的转录模块组成的ERF 108-RafS的基础冷诱导的棉子糖积累在植物中。
Ethylene-responsive factors (ERFs) are plant-specific transcription factors involved in cold stress response, and raffinose is known to accumulate in plants exposed to cold. However, it remains elusive whether ERFs function in cold tolerance by modulating raffinose synthesis. Here, we identified a cold-responsive PtrERF108 from trifoliate orange (Poncirus trifoliata (L.) Raf.), a cold-tolerant plant closely related to citrus. PtrERF108 is localized in the nucleus and has transcriptional activation activity. Overexpression of PtrERF108 conferred enhanced cold tolerance of transgenic lemon, whereas virus-induced gene silencing (VIGS)-mediated knockdown of PtrERF108 in trifoliate orange greatly elevated cold sensitivity. Transcriptome profiling showed that PtrERF108 overexpression caused extensive reprogramming of genes associated with signaling transduction, physiological processes and metabolic pathways. Among them, a raffinose synthase (RafS)-encoding gene, PtrRafS, was confirmed as a direct target of PtrERF108. RafS activity and raffinose content were significantly increased in PtrERF108-overexpressing transgenic plants, but prominently decreased in the VIGS plants under cold conditions. Meanwhile, exogenous replenishment of raffinose could recover the cold tolerance of PtrERF108-silenced plants, whereas VIGS-mediated knockdown of PtrRafS resulted in cold-sensitive phenotype. Taken together, the current results demonstrate that PtrERF108 plays a positive role in cold tolerance by modulation of raffinose synthesis via regulating PtrRafS. Our findings reveal a new transcriptional module composed of ERF108-RafS underlying cold-induced raffinose accumulation in plants.