Differential Dopamine Regulation of Ca(2+) Signaling and Its Timing Dependence in the Nucleus Accumbens.
Differential Dopamine Regulation of Ca(2+) Signaling and Its Timing Dependence in the Nucleus Accumbens.
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伏核中 Ca(2) 信号传导的多巴胺差异调节及其时间依赖性。
DOI：
10.1016/j.celrep.2016.03.055
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发表时间：
2016
期刊：
影响因子：
8.8
通讯作者：
Morikawa,Hitoshi
中科院分区：
文献类型：
--
作者：
Swapna,Immani;Bondy,Brian;Morikawa,Hitoshi
多巴胺在丘脑核（NAc）中的作用被认为是驱动食欲行为和巴甫洛夫奖励学习。然而，多巴胺如何通过调节NAc的中型多刺投射神经元（MSN）来实现这些行为效应，特别是在行为相关的时间尺度上，仍然存在争议。代谢型谷氨酸受体（mGluR）介导的依赖于释放Ca 2+的信使肌醇1，4，5-三磷酸（IP 3）的Ca 2+信号传导在控制神经元兴奋性和突触可塑性中起关键作用。在这里，我们表明，短暂的多巴胺的应用程序促进mGluR/IP 3诱导的Ca 2+信号的时间窗口内的102 -10秒的MSN在NAC核心的亚群。多巴胺易化IP 3诱导的Ca ~（2+）信号是由D1多巴胺受体介导的。在多巴胺不敏感的MSN中，A2 A腺苷受体的激活引起IP 3诱发的Ca 2+信号的增强，这被D2多巴胺受体激活逆转。这些结果表明，多巴胺差异调节Ca 2+信号的顺序秒在两个不同的MSN亚群。
Dopamine action in the nucleus accumbens (NAc) is thought to drive appetitive behavior and Pavlovian reward learning. However, it remains controversial how dopamine achieves these behavioral effects by regulating medium spiny projection neurons (MSNs) of the NAc, especially on a behaviorally relevant timescale. Metabotropic glutamate receptor (mGluR)-induced Ca2+signaling dependent on the Ca2+- releasing messenger inositol 1,4,5-triphosphate (IP3) plays a critical role in controlling neuronal excitability and synaptic plasticity. Here, we show that transient dopamine application facilitates mGluR/IP3-induced Ca2+signals within a time window of ∼2–10 s in a subpopulation of MSNs in the NAc core. Dopamine facilitation of IP3-induced Ca2+signaling is mediated by D1 dopamine receptors. In dopamine-insensitive MSNs, activation of A2A adenosine receptors causes enhancement of IP3-evoked Ca2+signals, which is reversed by D2 dopamine receptor activation. These results show that dopamine differentially regulates Ca2+signaling on the order of seconds in two distinct MSN subpopulations.