Sequence conservation and distribution of the fusobacterial immunosuppressive protein gene, fipA.
Sequence conservation and distribution of the fusobacterial immunosuppressive protein gene, fipA.
复制标题
梭杆菌免疫抑制蛋白基因 fipA 的序列保守和分布。
DOI：
10.1034/j.1399-302x.2002.170509.x
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发表时间：
2002
影响因子：
--
通讯作者：
Haake,SKinder
中科院分区：
文献类型：
--
作者：
Gerardo,SHunt;Yoder,SC;Citron,DM;Goldstein,EJC;Haake,SKinder
Fusobacterium nucleatumis a gram‐negative anaerobe involved in various diseases, including periodontitis. Recently, other investigators isolated theF. nucleatumFDC 364 fusobacterial immunosuppressive protein (FIP). One subunit, FipA, impairs T‐cell activationin vitroand shows homology with beta‐ketothiolases. However, its distribution and variability among fusobacteria was not reported. ClonedfipA gene sequences fromF. nucleatumssp.polymorphum(ATCC 10953) andF. nucleatumssp.nucleatum(ATCC 23726) shared 89 and 92% identity, respectively, with FDC 364fipA, and 90 and 94% identity, respectively, with the FDC 364 FipA predicted amino acid sequence. Southern blot analyses of chromosomal DNA from fusobacterial strains, includingF. nucleatumand otherFusobacteriumspecies, were performed using partialfipA sequences as probes. The results indicate thatfipA is highly conserved among theF. nucleatumstrains examined and thatfipA homologues are widely distributed among fusobacteria. A clear relationship between immune suppression, metabolism and the FipA protein remains to be determined.
