Kill and cure: genomic phylogeny and bioactivity of Burkholderia gladioli bacteria capable of pathogenic and beneficial lifestyles.
Kill and cure: genomic phylogeny and bioactivity of Burkholderia gladioli bacteria capable of pathogenic and beneficial lifestyles.
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DOI：
10.1099/mgen.0.000515
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发表时间：
2021-01
影响因子：
3.9
通讯作者：
Mahenthiralingam E
中科院分区：
文献类型：
--
作者：
Jones C;Webster G;Mullins AJ;Jenner M;Bull MJ;Dashti Y;Spilker T;Parkhill J;Connor TR;LiPuma JJ;Challis GL;Mahenthiralingam E
剑叶伯克霍尔德氏菌是一种具有广泛生态的细菌，跨越人类、动物和植物的疾病，但也包含多种有益的相互作用。它是一种植物病原体，是一种产生毒素的食物中毒因子，会导致囊性纤维化患者的肺部感染。与之形成鲜明对比的有益特征包括昆虫为保护其卵而利用的抗真菌生产、植物保护能力和抗生素的生物合成。我们探索了206个唐葛兰菌株的基因组多样性和专化代谢潜力，从系统发育上划分了5个分支。病史致病变种(PV.)唐葛兰属植物。大蒜和剑叶大蒜属(B.angdioli)椰子胶明显，而唐葛兰假单胞菌则不同。唐葛兰和唐葛兰属植物。蘑菇是难以区分的；软腐病和病原菌侵染在所有病型中都是保守的。毒黄素、甘草素和安息香素的生物合成基因簇(BGC)广泛分布于唐松子草中，而龙舌兰酸和唐葛兰素的合成是树枝专一性的。引人注目的是，13个 %的CF感染菌株呈Bongkrekic酸阳性，这是唯一将这种食物中毒毒素与唐葛兰芽孢杆菌病的这一方面联系在一起的。绘制唐氏假单胞菌的种群生物学和代谢物产生图揭示了其多样化的生态，并通过证明抗生素甲氧苄啶抑制bongkrekic酸的产生，确定了一种潜在的治疗策略，以将CF的中毒风险降至最低。
Burkholderia gladioli is a bacterium with a broad ecology spanning disease in humans, animals and plants, but also encompassing multiple beneficial interactions. It is a plant pathogen, a toxin-producing food-poisoning agent, and causes lung infections in people with cystic fibrosis (CF). Contrasting beneficial traits include antifungal production exploited by insects to protect their eggs, plant protective abilities and antibiotic biosynthesis. We explored the genomic diversity and specialized metabolic potential of 206 B. gladioli strains, phylogenomically defining 5 clades. Historical disease pathovars (pv.) B. gladioli pv. allicola and B. gladioli pv. cocovenenans were distinct, while B. gladioli pv. gladioli and B. gladioli pv. agaricicola were indistinguishable; soft-rot disease and CF infection were conserved across all pathovars. Biosynthetic gene clusters (BGCs) for toxoflavin, caryoynencin and enacyloxin were dispersed across B. gladioli , but bongkrekic acid and gladiolin production were clade-specific. Strikingly, 13 % of CF infection strains characterized were bongkrekic acid-positive, uniquely linking this food-poisoning toxin to this aspect of B. gladioli disease. Mapping the population biology and metabolite production of B. gladioli has shed light on its diverse ecology, and by demonstrating that the antibiotic trimethoprim suppresses bongkrekic acid production, a potential therapeutic strategy to minimize poisoning risk in CF has been identified.
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影响因子：
30.8
作者：
通讯作者：
--
影响因子：
3.5
作者：
Estrada-de Los Santos P;Palmer M;Chávez-Ramírez B;Beukes C;Steenkamp ET;Briscoe L;Khan N;Maluk M;Lafos M;Humm E;Arrabit M;Crook M;Gross E;Simon MF;Dos Reis Junior FB;Whitman WB;Shapiro N;Poole PS;Hirsch AM;Venter SN;James EK
通讯作者：
James EK
影响因子：
4.4
作者：
Chowdhury, Piklu Roy;Heinemann, Jack A.
通讯作者：
Heinemann, Jack A.
影响因子：
3.9
作者：
Connor TR;Loman NJ;Thompson S;Smith A;Southgate J;Poplawski R;Bull MJ;Richardson E;Ismail M;Thompson SE;Kitchen C;Guest M;Bakke M;Sheppard SK;Pallen MJ
通讯作者：
Pallen MJ
影响因子：
2.8
作者：
Gill, WM;Tsuneda, A
通讯作者：
Tsuneda, A