A COLCEMID-SENSITIVE MECHANISM INVOLVED IN REGULATION OF CHROMOSOME MOVEMENTS DURING MEIOTIC PAIRING
A COLCEMID-SENSITIVE MECHANISM INVOLVED IN REGULATION OF CHROMOSOME MOVEMENTS DURING MEIOTIC PAIRING
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DOI：
10.1007/bf00330775
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发表时间：
1982-01-01
期刊：
影响因子：
1.6
通讯作者：
PARVINEN, M
中科院分区：
文献类型：
--
作者：
SALONEN, K;PARANKO, J;PARVINEN, M
在减数分裂配对过程中，同源染色体找到彼此的过程中可能需要染色体的活跃运动。由于细胞骨架的组成通常被认为负责活的非肌肉细胞中的所有运动，因此通过使用细胞骨架各组成部分组装的特定抑制剂来分析雄性大鼠合线染色体运动的调节。微管形成的抑制剂Colcemid在1ug/ml的浓度下可完全抑制体外染色体的运动，这与EM分析显示的核被膜损伤有关。另一种微管形成的抑制剂，长春花碱，在一般毒性水平(100微克/毫升)以下无效。一种特殊的微丝抑制剂，细胞松弛素B，也同样无效。合子期精母细胞的核膜中存在一种特殊的秋水线虫敏感机制，该机制调节减数分裂配对过程中染色体的运动。
Active movements of the chromosomes may be needed in the process where homologous chromosomes find each other during meiotic pairing. Because the components of the cytoskeleton are generally believed to be responsible for all movements in living nonmuscle cells, the regulation of the movements of zygotene chromosomes in the male rat was analyzed by using specific inhibitors of the assembly of the various components of the cytoskeleton. Colcemid, an inhibitor of microtubule formation, completely inhibited the chromosome movements in vitro at a concentration of 1 .mu.g/ml. This was associated with damage of the nuclear envelope revealed by EM analysis. Another inhibitor of microtubule formation, vinblastine, was ineffective below the level of general toxicity (100 .mu.g/ml). A specific microfilament inhibitor, cytochalasin B, was similarly ineffective. The presence of a specific colcemid-sensitive mechanism is suggested in the nuclear envelope of the zygotene spermatocytes, which regulates the movements of the chromosomes during meiotic pairing.