Phylogenetic signal of host plants in the bacterial and fungal root microbiomes of cultivated angiosperms
Phylogenetic signal of host plants in the bacterial and fungal root microbiomes of cultivated angiosperms
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DOI：
10.1111/tpj.14943
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发表时间：
2020-08-25
期刊：
影响因子：
7.2
通讯作者：
Sugiyama, Shuichi
中科院分区：
文献类型：
--
作者：
Wang, Boxi;Sugiyama, Shuichi
根系微生物群是通过寄主植物从潜在伙伴中选择性招募而建立的。然而，根系微生物组的组装规则仍不清楚。为了阐明寄主植物系统发育对根系微生物组组成的影响，以及哪些微生物群影响根系微生物组组成的差异，本研究比较了20种栽培被子植物的细菌和真菌根系微生物组的结构。每个物种的表面灭菌种子播种在相同的土壤中，7-8周后从植物根部提取DNA。然后使用Illumina MiSeq检测细菌（16S rRNA）和真菌（ITS）群落。寄主植物的系统发育距离与细菌微生物组的组装差异显著相关，而真菌微生物组的组装差异不显著，寄主植物系统发育树和群落不相似树的拓扑结构也显著相关，从而证实了细菌根微生物组的系统发育保守性。此外，寄主植物系统发育主要影响少数特定的细菌谱系，包括Betaproteobacteria， Gammaproteobacteria和Chloroflexi。伯克霍尔德菌属（Betaproteobacteria）在单子型中数量多于双子型，而链霉菌属（streptomyces）在单子型中数量较少。这些发现表明，细菌根微生物组对被子植物在较高分类水平上的功能分化起着重要作用。
Root microbiomes are established through selective recruitment by host plants from pools of potential partners. However, the assembly rules of root microbiomes remain unclear. To elucidate (i) the effects of host plant phylogeny on root microbiome assembly and (ii) which microbial groups affect differences in root microbiome assemblies, the structures of bacterial and fungal root microbiomes from 20 cultivated angiosperms were compared. Surface-sterilized seeds from each species were sown in identical soil, and DNA was extracted from the plant roots after 7-8 weeks. The bacterial (16S rRNA) and fungal (ITS) communities were then examined using Illumina MiSeq. The phylogenetic distances of host plants and assembly dissimilarities of bacterial microbiomes, but not of fungal ones, were significantly correlated, as were the topologies of the host plant phylogenetic tree and the community dissimilarity tree, thereby confirming the phylogenetic conservation of bacterial root microbiomes. Furthermore, host plant phylogeny mainly affected only a few specific bacterial lineages, including the Betaproteobacteria, Gammaproteobacteria, and Chloroflexi.Burkholderia(Betaproteobacteria) taxa were more abundant in monocots than in dicots, whereasStreptomyces(Actinobacteria) taxa were less abundant. These findings suggest that bacterial root microbiomes have significantly contributed to the functional divergence of angiosperms at higher taxonomic levels.