Reduced graphene oxide-silver nanoparticle nanocomposite: a potential anticancer nanotherapy.
Reduced graphene oxide-silver nanoparticle nanocomposite: a potential anticancer nanotherapy.
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DOI：
10.2147/ijn.s92449
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发表时间：
2015
影响因子：
8
通讯作者：
Kim JH
中科院分区：
文献类型：
--
作者：
Gurunathan S;Han JW;Park JH;Kim E;Choi YJ;Kwon DN;Kim JH
石墨烯和石墨烯基纳米复合材料用于各种研究领域，包括传感，储能和催化。机械、热、电和生物学性质使得金属纳米颗粒的石墨烯基纳米复合材料可用于若干生物医学应用。上皮性卵巢癌是女性第五大致命癌症;大多数肿瘤最初对化疗有反应，但最终获得耐药性。因此，开发用于癌症治疗的新分子是必不可少的。本研究旨在开发一种简单、无毒、环境友好的方法，以紫椴（Tilia amurensis）植物提取物为还原剂和稳定剂，合成还原氧化石墨烯-银（rGO-Ag）纳米颗粒复合材料。在卵巢癌细胞中评价rGO-Ag的抗癌特性。合成的rGO-Ag纳米复合材料，其特征在于使用各种分析技术。rGO-Ag纳米复合物的抗癌特性使用一系列测定法进行评价，例如细胞活力、乳酸脱氢酶渗漏、活性氧物质产生、丙二醛和谷胱甘肽的细胞水平、半胱天冬酶-3活性和卵巢癌细胞（A2780）中的DNA片段化。平均尺寸为20 nm的AgNP均匀分散在石墨烯片上。从生物化学测定中获得的数据表明，与其他测试的纳米材料如氧化石墨烯、rGO和AgNP相比，rGO-Ag纳米复合材料显著抑制A2780卵巢癌细胞中的细胞活力，并增加乳酸脱氢酶泄漏、活性氧物质产生、半胱天冬酶-3活性和DNA片段化。T.结果表明，紫茎泽兰植物提取物介导的rGO-Ag纳米复合材料可促进石墨烯基纳米复合材料的大规模生产;与氧化石墨烯、rGO和银纳米颗粒相比，rGO-Ag对细胞活力显示出显著的抑制作用。该纳米复合材料可以成为治疗癌症和癌症干细胞的有效无毒治疗剂。
Graphene and graphene-based nanocomposites are used in various research areas including sensing, energy storage, and catalysis. The mechanical, thermal, electrical, and biological properties render graphene-based nanocomposites of metallic nanoparticles useful for several biomedical applications. Epithelial ovarian carcinoma is the fifth most deadly cancer in women; most tumors initially respond to chemotherapy, but eventually acquire chemoresistance. Consequently, the development of novel molecules for cancer therapy is essential. This study was designed to develop a simple, non-toxic, environmentally friendly method for the synthesis of reduced graphene oxide–silver (rGO–Ag) nanoparticle nanocomposites using Tilia amurensis plant extracts as reducing and stabilizing agents. The anticancer properties of rGO–Ag were evaluated in ovarian cancer cells. The synthesized rGO–Ag nanocomposite was characterized using various analytical techniques. The anticancer properties of the rGO–Ag nanocomposite were evaluated using a series of assays such as cell viability, lactate dehydrogenase leakage, reactive oxygen species generation, cellular levels of malonaldehyde and glutathione, caspase-3 activity, and DNA fragmentation in ovarian cancer cells (A2780). AgNPs with an average size of 20 nm were uniformly dispersed on graphene sheets. The data obtained from the biochemical assays indicate that the rGO–Ag nanocomposite significantly inhibited cell viability in A2780 ovarian cancer cells and increased lactate dehydrogenase leakage, reactive oxygen species generation, caspase-3 activity, and DNA fragmentation compared with other tested nanomaterials such as graphene oxide, rGO, and AgNPs. T. amurensis plant extract-mediated rGO–Ag nanocomposites could facilitate the large-scale production of graphene-based nanocomposites; rGO–Ag showed a significant inhibiting effect on cell viability compared to graphene oxide, rGO, and silver nanoparticles. The nanocomposites could be effective non-toxic therapeutic agents for the treatment of both cancer and cancer stem cells.