Micromanipulation of Langmuir-monolayers with optical tweezers.

Micromanipulation of Langmuir-monolayers with optical tweezers.
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用光镊对朗缪尔单层进行显微操作。

DOI:
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发表时间:
2001
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影响因子:
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通讯作者:
T. Fischer
T. Fischer
中科院分区:
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文献类型:
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作者:
S. Wurlitzer;C. Lautz;M. Liley;C. Duschl;T. Fischer

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提出了一种在空气/水界面上操纵朗缪尔单分子膜的技术。光镊与荧光和布鲁斯特角显微镜相结合,以产生一个系统,能够在微米尺度上的单分子层的机械和热处理。的粘弹性,电气和热力学性能的单层可以通过分析其结构的扰动。给出了镊子的力热标定方法。这两种显微操作都应用于双[8-(1,2-二棕榈酰-sn-甘油-3-磷酰基)-3,6-二氧杂辛基]二硫化物("硫脂")单分子膜的液体膨胀环境中的星星形凝聚域。研究了恒星的稳定性及其在界面流上互锁的后果。
A technique for the manipulation of Langmuir-monolayers at the air/water interface is presented. Optical tweezers are combined with fluorescence and Brewster angle microscopy to produce a system capable of mechanical and thermal handling of monolayers on a micrometer scale. The viscoelastic, electrical, and thermodynamic properties of the monolayer can be determined by analysis of the perturbations of its structure. A method for force and heat calibration of the tweezers is given. Both kinds of micromanipulations are applied to the star shaped condensed domains in liquid expanded surroundings of bis[8-(1,2-dipalmitoyl-sn-glycero-3-phosphoryl)-3,6-dioxaoctyl]disulfide (“thiolipid”) monolayers. The stability of the stars and the consequence of their interlocking on the interfacial flow are investigated.