Susceptibility of a North American Culex quinquefasciatus to Japanese Encephalitis Virus
Susceptibility of a North American Culex quinquefasciatus to Japanese Encephalitis Virus
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DOI：
10.1089/vbz.2015.1821
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发表时间：
2015-11-01
影响因子：
2.1
通讯作者：
Vanlandingham, Dana L.
中科院分区：
文献类型：
--
作者：
Huang, Yan-Jang S.;Harbin, Julie N.;Vanlandingham, Dana L.
日本脑炎病毒（Japanese encephalitis virus，JEV）是一种由库蚊（Culex，Cx.）亚洲热带和亚热带地区的三带喙龙。地方性传播循环涉及充当扩增宿主的家猪和禽类物种;人类是偶然宿主，不能发展足以引起蚊子感染的高滴度病毒血症。虽然疫苗接种可以是一种有效的疾病预防策略，并在多个亚洲国家广泛使用，但未接种疫苗的免疫幼稚人群可能会出现严重的神经系统后遗症。JEV可能传入北美将对人类和动物健康构成重大威胁。在这项研究中，现场收集的Cx。来自格鲁吉亚的瓦尔多斯塔的致倦犬对JEV的敏感性和通过经口感染发展为播散性感染的潜力进行了测试。这些结果表明，北美Cx。致倦犬对JEV感染和随后在感染后14天（d.p.i.）的传播易感。从受感染蚊子唾液中检测到的病毒RNA也表明在北美可以发现JEV的胜任载体。
Japanese encephalitis virus (JEV) is a flavivirus that is transmitted by Culex (Cx.) tritaeniorhynchus in tropical and subtropical regions of Asia. The endemic transmission cycle involves domestic pigs and avian species that serve as amplification hosts; humans are incidental hosts that cannot develop a high-titer viremia sufficient for mosquito infection. Although vaccination can be an effective strategy for disease prevention and is used extensively in multiple Asian countries, unvaccinated immunologically naive human populations can suffer from severe neurological sequelae. The potential introduction of JEV into North America would be a major threat to human and animal health. In this study, field-collected Cx. quinquefasciatus from Valdosta, Georgia, were tested for their susceptibility to JEV and their potential to develop a disseminated infection via per os infection. These results demonstrate that North American Cx. quinquefasciatus are susceptible to JEV infection and subsequent dissemination at 14 days post infection (d.p.i.). Detection of viral RNA in saliva from infected mosquitoes also indicates competent vectors for JEV can be found in North America.