RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta
RNA-seq analysis provides insight into reprogramming of culm development in Zizania latifolia induced by Ustilago esculenta
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RNA-seq 分析提供了对黑粉菌诱导的茭白秆发育重编程的见解
DOI：
10.1007/s11103-017-0658-9
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
De‑Ping Guo
中科院分区：
文献类型：
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作者：
Zhi‑Dan Wang;Ning Yan;Zheng‑Hong Wang;Xiao‑Huan Zhang;Jing‑Ze Zhang;Hui‑Min Xue;Li‑Xia Wang;Qi Zhan;Ying‑Ping Xu;De‑Ping Guo
关键信息我们报道了来自RNA-Seq数据的转录组组装和表达谱，并鉴定了由黑粉菌Ustilago esculentacan诱导的宽叶Zizania茎瘤形成的相关基因。摘要黑粉菌Ustilago esculentacan在亚洲国家作为蔬菜使用时，可以诱导宽Zizania茎瘤。然而，茎瘤形成的潜在分子机制仍不清楚。为了表征这种寄主-真菌关联的过程，我们对来自Z的秸秆进行了转录和表达谱分析。受真菌U感染的阔叶树。美食家。转录分析检测到dU。诱导角白型(JB)和徽角型(HJ)胆汁中19,033个和17,669个基因的差异表达。此外，为了检测潜在的根瘤诱导基因，还分析了在茎瘤发育的−7、1和10 DAS收集的感染茎秆的表达谱。与对照相比，我们分别检测到8089个基因(4389个上调，3700个下调)和5251个基因(3121个上调，2130个下调)在JB和HJ中差异表达。我们鉴定了376个寄主和187个真菌候选基因，这些基因表现出阶段特异性的表达模式，它们可能分别在初期和后期负责胆汁的形成。我们的结果表明，细胞分裂素在调节胆汁形成方面的作用比生长素更显著。总之，我们的工作为理解茎瘤发育的基因调控网络提供了一般性的启示。宽叶，以及遗传操作的潜在目标，以提高这种作物未来的产量。
Key messageWe report a transcriptome assembly and expression profiles from RNA-Seq data and identify genes responsible for culm gall formation inZizania latifoliainduced byUstilago esculenta.AbstractThe smut fungusUstilago esculentacan induce culm gall inZizania latifolia, which is used as a vegetable in Asian countries. However, the underlying molecular mechanism of culm gall formation is still unclear. To characterize the processes underlying this host-fungus association, we performed transcriptomic and expression profiling analyses of culms fromZ. latifoliainfected by the fungusU. esculenta. Transcriptomic analysis detectedU. esculentainduced differential expression of 19,033 and 17,669 genes inJiaobai(JB) andHuijiao(HJ) type of gall, respectively. Additionally, to detect the potential gall inducing genes, expression profiles of infected culms collected at −7, 1 and 10 DAS of culm gall development were analyzed. Compared to control, we detected 8089 genes (4389 up-regulated, 3700 down-regulated) and 5251 genes (3121 up-regulated, 2130 down-regulated) were differentially expressed in JB and HJ, respectively. And we identified 376 host and 187 fungal candidate genes that showed stage-specific expression pattern, which are possibly responsible for gall formation at the initial and later phases, respectively. Our results indicated that cytokinins play more prominent roles in regulating gall formation than do auxins. Together, our work provides general implications for the understanding of gene regulatory networks for culm gall development inZ. latifolia, and potential targets for genetic manipulation to improve the future yield of this crop.