A potassium channel protein encoded by chlorella virus PBCV-1
A potassium channel protein encoded by chlorella virus PBCV-1
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DOI：
10.1126/science.287.5458.1641
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发表时间：
2000-03-03
期刊：
影响因子：
56.9
通讯作者：
Thiel, G
中科院分区：
文献类型：
--
作者：
Plugge, B;Gazzarrini, S;Thiel, G
大小球藻病毒 PBCV-1 含有双链 DNA (dsDNA)，编码 94 个密码子开放阅读框 (ORF)，其中包含类似于钾通道蛋白孔结构域特征序列的基序。所编码的蛋白质 Kcv 的系统发育分析表明了一种先前未识别的钾通道类型。 ORF 编码的信使 RNA 导致非洲爪蟾卵母细胞中钾选择性电导的功能性表达。除了病毒空斑的形成之外，通道阻滞剂金刚烷胺和钡（但不是铯）也能抑制这种电导。因此，PBCV-1 编码第一个已知的病毒蛋白，该蛋白充当钾选择性通道，并且在病毒生命周期中至关重要。
The Large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF Leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus Life cycle.