The glial and mesenchymal elements of gliosarcomas share similar genetic alterations
The glial and mesenchymal elements of gliosarcomas share similar genetic alterations
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DOI：
10.1097/00005072-199609000-00004
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发表时间：
1996-09-01
影响因子：
3.2
通讯作者：
Jenkins, RB
中科院分区：
文献类型：
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作者：
Boerman, RH;Anderl, K;Jenkins, RB
胶质肉瘤的肉瘤成分的细胞起源是有争议的。目前尚不清楚肉瘤是由包含胶质瘤成分的胶质细胞转变而来，还是独立由肿瘤基质的非肿瘤性间充质细胞产生。利用比较基因组杂交（CGH）、细胞遗传学分析、荧光原位杂交（FISH）分析和聚合酶链反应（PCR）分析微卫星等位基因失衡，我们评估了5种胶质肉瘤胶质瘤和肉瘤成分的遗传改变。所有5个肿瘤的胶质成分均为4级原纤维星形细胞瘤（多形性胶质母细胞瘤）。肉瘤成分为成纤维细胞，无骨性、软骨样或血管肉瘤分化。7号染色体的增加、10号染色体的缺失、9号染色体p臂的缺失和3号染色体的改变经常被观察到，这表明胶质肉瘤可以在遗传学上归类为胶质母细胞瘤。此外，所研究的每个胶质肉瘤的肉瘤和胶质瘤部分在存在和不存在特定遗传改变方面是相似的。这一观察结果支持了一种假设，即胶质瘤的肉瘤成分要么来自与胶质瘤部分相同的前体细胞，要么来自胶质瘤部分本身。
The cellular origin of the sarcomatous component of gliosarcomas is controversial. it is not clear if the sarcoma arises in transition from the glial cells that comprise the gliomatous component or independently arises from non-neoplastic mesenchymal cells of the tumor stroma. Using comparative genomic hybridization (CGH) along with cytogenetic analysis, fluorescence in situ hybridization (FISH) analysis, and polymerase chain reaction (PCR) analysis of microsatellite allelic imbalance, we have evaluated the genetic alterations in the gliomatous and sarcomatous components of five gliosarcomas. The glial element was grade 4 fibrillary astrocytoma (glioblastoma multiforme) in all five tumors. The sarcoma elements were fibroblastic without osseous, chondroid, or angiosarcomatous differentiation. Gain of chromosome 7, loss of chromosome 10, deletions of the chromosome 9 p-arm, and alterations of chromosome 3 were frequently observed, demonstrating that gliosarcomas can be genetically classified as belonging to the spectrum of glioblastomas. Furthermore, the sarcomatous and gliomatous portions of each gliosarcoma investigated were similar with respect to both the presence and absence of specific genetic alterations. This observation supports the hypothesis that the sarcomatous component of a gliosarcoma either arises from the same common precursor cell as the gliomatous portion, or it arises from the gliomatous portion itself.