Circovirus in tissues of dogs with vasculitis and hemorrhage.
Circovirus in tissues of dogs with vasculitis and hemorrhage.
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DOI：
10.3201/eid1904.121390
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发表时间：
2013-04
影响因子：
11.8
通讯作者：
Pesavento PA
中科院分区：
文献类型：
--
作者：
Li L;McGraw S;Zhu K;Leutenegger CM;Marks SL;Kubiski S;Gaffney P;Dela Cruz FN Jr;Wang C;Delwart E;Pesavento PA
我们从患有严重出血性胃肠炎、血管炎和肉芽肿性淋巴结炎的狗的肝脏中鉴定了一种新型狗圆环病毒（DogCV）的完整基因组。通过PCR在19/168（11.3%）只腹泻犬和14/204（6.9%）只健康犬的粪便样本中以及19/409（3.3%）只血小板减少症和中性粒细胞减少症、不明原因发热或既往蜱虫叮咬犬的血液中检测到DogCV。在13/19（68%）只DogCV阳性腹泻犬中检测到与其他犬病原体的合并感染。来自不同狗的DogCV衣壳蛋白变化高达8%。原位杂交和透射电镜检测DogCV在淋巴结和脾脏的4只狗血管妥协和组织细胞炎症。在犬组织中检测到圆环病毒，扩大了这些病毒对第二种哺乳动物宿主的已知嗜性。我们的研究结果表明，圆环病毒，单独或与其他病原体的共同感染，可能有助于疾病和死亡的狗。
We characterized the complete genome of a novel dog circovirus (DogCV) from the liver of a dog with severe hemorrhagic gastroenteritis, vasculitis, and granulomatous lymphadenitis. DogCV was detected by PCR in fecal samples from 19/168 (11.3%) dogs with diarrhea and 14/204 (6.9%) healthy dogs and in blood from 19/409 (3.3%) of dogs with thrombocytopenia and neutropenia, fever of unknown origin, or past tick bite. Co-infection with other canine pathogens was detected for 13/19 (68%) DogCV-positive dogs with diarrhea. DogCV capsid proteins from different dogs varied by up to 8%. In situ hybridization and transmission electron microscopy detected DogCV in the lymph nodes and spleens of 4 dogs with vascular compromise and histiocytic inflammation. The detection of a circovirus in tissues of dogs expands the known tropism of these viruses to a second mammalian host. Our results indicate that circovirus, alone or in co-infection with other pathogens, might contribute to illness and death in dogs.