Co-expression changes of lncRNAs and mRNAs in the cervical sympathetic ganglia in diabetic cardiac autonomic neuropathic rats
Co-expression changes of lncRNAs and mRNAs in the cervical sympathetic ganglia in diabetic cardiac autonomic neuropathic rats
复制标题
糖尿病心脏自主神经病变大鼠颈交感神经节 lncRNA 和 mRNA 的共表达变化。
DOI：
10.1002/jnr.24000
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发表时间：
2017-08-01
影响因子：
4.2
通讯作者：
Zhu, Gaochun
中科院分区：
文献类型：
--
作者：
Li, Guilin;Sheng, Xuan;Zhu, Gaochun
2型糖尿病心脏自主神经病变(T2D)通常是一种毁灭性的并发症。长非编码RNAs(Long Non-Coding RNAs，LncRNAs)在正常发育和疾病发病机制中都具有重要作用。在这项研究中，我们探索了一些参与炎症的lncRNAs在2型糖尿病损伤后的颈上神经节和星状神经节中可能与信使RNA(MRNA)共表达的表达谱。从10对糖尿病和正常雄性大鼠颈上神经节和星状神经节中提取总RNA，与lncRNA阵列杂交进行检测。通路分析表明，在糖尿病中上调的最重要的基因本体论(GO)过程与免疫反应、细胞迁移、防御反应、趋化和趋化相关。京都基因和基因组百科全书(KEGG)显示，LncRNAs的靶基因大多位于细胞因子-细胞因子受体相互作用、趋化因子信号通路和细胞黏附分子中，参与T2D。基因共表达网络的构建表明，实验大鼠的共表达网络由105个lncRNAs和11个mRNAs中的268条调控边组成。我们的研究证实了lncRNAs和mRNAs在糖尿病心脏自主神经节中的共表达情况，提示多个lncRNAs可能作为开发糖尿病心脏自主神经病变的治疗策略或生物标志物的潜在靶点。(C)2016威利期刊公司。
Cardiac autonomic neuropathy in Type 2 diabetes (T2D) is often a devastating complication. Long non-coding RNAs (lncRNAs) have important effects on both normal development and disease pathogenesis. In this study, we explored the expression profiles of some lncRNAs involved in inflammation which may be co-expressed with messenger RNA (mRNA) in superior cervical and stellate ganglia after type 2 diabetic injuries. Total RNA isolated from 10 pairs of superior cervical and stellate ganglia in diabetic and normal male rats was hybridized to lncRNA arrays for detections. Pathway analysis indicated that the most significant gene ontology (GO) processes that were upregulated in diabetes were associated with immune response, cell migration, defense response, taxis, and chemotaxis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that most of the target genes of the lncRNAs were located in cytokine-cytokine receptor interactions, the chemokine signaling pathway and cell adhesion molecules, which were involved in T2D. Gene co-expression network construction showed that the co-expression network in the experimental rats consisted of 268 regulation edges among 105 lncRNAs and 11 mRNAs. Our studies demonstrated the co-expression profile of lncRNAs and mRNAs in diabetic cardiac autonomic ganglia, suggesting possible roles for multiple lncRNAs as potential targets for the development of therapeutic strategies or biomarkers for diabetic cardiac autonomic neuropathy. (c) 2016 Wiley Periodicals, Inc.