Characterization and biocompatibility studies of novel humic acids based films as membrane material for an implantable glucose sensor
Characterization and biocompatibility studies of novel humic acids based films as membrane material for an implantable glucose sensor
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DOI：
10.1021/bm010112y
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发表时间：
2001-12-01
影响因子：
6.2
通讯作者：
Papadimitrakopoulos, F
中科院分区：
文献类型：
--
作者：
Galeska, I;Hickey, T;Papadimitrakopoulos, F
研究了腐植酸（HAs）（天然存在的生物聚合物）多层膜作为植入式葡萄糖传感器的潜在半透膜。这些薄膜是利用羟基磷灰石和带相反电荷的铁离子的一层一层的自组装过程生长的。这些组装体的生长对HAs溶液的pH和离子强度有很强的依赖性，这与羧基的电离程度和中和引起的表面扩散有关。石英晶体微天平（QCM）和椭偏研究表明，这些组件的质量（高达5.63马克杯/厘米(2)）和膜厚度（每层约24.3纳米）可重复地逐步增加。葡萄糖通过这些膜的渗透性可以通过改变自组装的HAs/Fe3+ C层的数量来调节。此外，200 nm厚的HAs/Fe3+膜（水合态）的剪切模量约为80 MPa，表明注入后具有稳定性。这些膜被确定为生物相容性，因为体内研究表明只有轻微的组织反应和一些新生血管。
Multilayered films of humic acids (HAs) (naturally occurring biopolymers) were investigated as a potential semipermeable membrane for implantable glucose sensors. These films were grown using a layer-by-layer self-assembly process of HAs and oppositely charged ferric ions. The growth of these assemblies exhibited strong dependence on the pH and ionic strength of HAs solutions, which correlated with the degree of ionization of the carboxyl groups and neutralization-induced surface spreading. Quartz crystal microbalance (QCM) and ellipsometric studies have shown repeatable, stepwise increase in mass (as high as 5.63 mug/cm(2)) and in film thickness (ca. 24.3 nm per layer) for these assemblies. The permeability of glucose through these membranes can be regulated by varying the number of self-assembled HAs/Fe3+ C layers. Moreover, a 200 nm thick HAs/Fe3+ film (in its hydrated state) had a shear modulus of about 80 MPa, implying stability upon implantation. These films were determined to be biocompatible since in vivo studies indicated only mild tissue reaction along with some neovascularization.