Directed differentiation of motor neuron cell-like cells from human adipose-derived stem cells in vitro
Directed differentiation of motor neuron cell-like cells from human adipose-derived stem cells in vitro
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DOI：
10.1097/wnr.0b013e3283469615
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发表时间：
2011-06
期刊：
影响因子：
1.7
通讯作者：
Yang Liqing;Geng Jia;Cao Jiqing;Gu Ran;Chen Fei;Kong Jie;Wang Yanyun;Zhang Cheng
中科院分区：
文献类型：
--
作者：
Yang Liqing;Geng Jia;Cao Jiqing;Gu Ran;Chen Fei;Kong Jie;Wang Yanyun;Zhang Cheng
人类脂肪源性干细胞（hADSCs）分化为运动神经元的能力以及赋予hADSCs运动神经元能力的分子因子的身份尚未阐明。本研究应用维甲酸和超音hedgehog基因检测hscs是否能分化为运动神经元。早在诱导后6 h， hascs就向神经元形态转变。诱导后，hscs免疫细胞化学染色显示- iii -微管蛋白、胆碱乙酰转移酶和神经元特异性烯醇化酶阳性。逆转录酶聚合酶链反应表征表明，从hascs分化的细胞仅限于脊柱腹侧命运（Nkx2.2, Pax6， Hb9和Olig2）。我们的研究结果表明，hascs可能是运动神经元疾病细胞治疗的潜在候选者。
The capacity of human adipose-derived stem cells (hADSCs) to differentiate into motor neurons and the identity of molecular factors that confer hADSCs with the competence of motor neurons have yet to be elucidated. Here, retinoic acid and sonic hedgehog were applied to examine whether hADSCs could be differentiated into motor neurons. As early as 6 h after induction, hADSCs were changed toward neuronal morphology. After induction, hADSCs showed positive immunocytochemical staining for -III-tubulin, choline acetyltransferase, and neuron-specific enolase. Reverse-transcriptase polymerase chain reaction characterization indicated that cells differentiated from hADSCs were restricted to the ventral spinal fate (Nkx2.2, Pax6, Hb9, and Olig2). Our results suggest that hADSCs may be a potential candidate in cellular therapy for motor neuron disease.