Dual AuNPs detecting probe enhanced the NanoSPR effect for the high-throughput detection of the cancer microRNA21 biomarker
Dual AuNPs detecting probe enhanced the NanoSPR effect for the high-throughput detection of the cancer microRNA21 biomarker
复制标题
双AuNPs检测探针增强了NanoSPR效应，用于癌症microRNA21生物标志物的高通量检测
DOI：
10.1016/j.bios.2023.115084
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发表时间：
2023-01-22
影响因子：
12.6
通讯作者：
Liu, Gang L.
中科院分区：
文献类型：
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作者：
Liu, Juxiang;Hu, Xulong;Liu, Gang L.
MicroRNA21（miR-21）是一种特定的肿瘤生物标志物，对于几种癌症类型的诊断至关重要，对其过表达模式的研究对于癌症诊断很重要。在此，我们报告了使用纳米质阵列芯片与金纳米颗粒（AUNPS）的纳米质阵列芯片检测MiR-21的低成本，快速，超敏感和方便的生物传感策略。该传感平台结合了纳米质学（NanoSPR）和局部表面等离子体等离子体谐振（LSPR）效应的表面等离子体的共振效应，从而可以实时监测靶向miRNA介绍过程中介电常数的变化引起的细微光密度（OD）变化。使用这种方法，miRNA的检测范围从100 am到1 mu m，并且检测限（LOD）为1.85 AM。此外，该测定法还具有单基分辨率来区分高度同源的miRNA。更重要的是，该平台具有较高的吞吐量特征（可以同时检测到96个样本）。与传统生物传感器相比，该策略在敏感性方面表现出了86.5倍以上。
The microRNA21 (miR-21), a specific tumor biomarker, is crucial for the diagnosis of several cancer types, and investigation of its overexpression pattern is important for cancer diagnosis. Herein, we report a low-cost, rapid, ultrasensitive, and convenient biosensing strategy for the detection of miR-21 using a nanoplasmonic array chip coupled with gold nanoparticles (AuNPs). This sensing platform combines the surface plasmon resonance effect of nanoplasmonics (NanoSPR) and the localized surface plasmon resonance (LSPR) effect, which allows the real-time monitoring of the subtle optical density (OD) changes caused by the variations in the dielectric constant in the process of the hybridization of the target miRNA. Using this method, the miRNA achieves a broad detection range from 100 aM to 1 mu M, and with a limit of detection (LoD) of 1.85 aM. Furthermore, this assay also has a single-base resolution to discriminate the highly homologous miRNAs. More importantly, this platform has high throughput characteristics (96 samples can be detected simultaneously). This strategy exhibits more than 86.5 times enhancement in terms of sensitivity compared to that of traditional biosensors.