CIRCULATING ANTIBODY TO PROSTATE ANTIGEN IN PATIENTS WITH PROSTATIC-CANCER
CIRCULATING ANTIBODY TO PROSTATE ANTIGEN IN PATIENTS WITH PROSTATIC-CANCER
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DOI：
10.1111/j.1749-6632.1983.tb32880.x
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发表时间：
1983-01-01
影响因子：
5.2
通讯作者：
WANG, MC
中科院分区：
文献类型：
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作者：
CHU, TM;KURIYAMA, M;WANG, MC
一种反向酶联免疫吸附测定 (ELISA) 是根据之前报道的前列腺抗原 (PA) 测定改进而来，用于测量循环 PA 结合球蛋白 (PABG)。对取自正常男性、正常女性、患有非前列腺癌的男性患者、良性前列腺肥大患者以及不同阶段的前列腺癌患者的血清标本进行PABG分析。结果显示，只有晚期前列腺癌患者的 PABG 水平才会升高。然后通过PA亲和层析从前列腺癌患者的血清中分离PABG。经过免疫扩散和免疫电泳，该 PABG 制剂与纯化的 PA、抗 PA 异种抗体和抗人 IgG 发生反应。通过免疫过氧化物酶技术，PABG 在前列腺导管上皮细胞中呈阳性染色，而在所有其他检查组织中呈阴性染色。另一种 PABG 制剂与抗 PA 和抗人 IgG 反应，但不与纯化的 PA 反应，也通过抗 PA 亲和层析分离。这些PABG制剂分别经过高效液相色谱进一步纯化。获得了Mr 大于240K、150K 和34K 的三个主要蛋白质峰。这些结果表明，与PA反应的循环IgG抗体存在于前列腺转移癌患者中，并且其部分以与PA复合的形式存在，并且与前列腺的导管上皮成分特异性反应。
A reverse enzyme-linked immunosorbent assay (ELISA) modified from a prostate antigen (PA) assay previously reported has been developed to measure circulating PA-binding globulin (PABG). Serum specimens taken from normal males, normal females, male patients with nonprostatic cancers, patients with benign prostatic hypertrophy, and patients with various stages of prostate cancer were analyzed for PABG. Results revealed that only patients with an advanced stage of prostatic cancer exhibited an elevated level of PABG. PABG was then isolated from prostatic cancer patients' serum by PA affinity chromatography. Upon immunodiffusion and immunoelectrophoresis, this PABG preparation reacted with purified PA, anti-PA xenoantibodies and anti-human IgG. By the immunoperoxidase technique, PABG stained positively in prostatic ductal epithelial cells and negatively in all other tissues examined. An additional PABG preparation, which reacted with anti-PA and anti-human IgG and not with purified PA, was also isolated by an anti-PA affinity chromatography. These PABG preparations were separately subjected to high-performance liquid chromatography for further purification. Three major protein peaks at Mr of greater than 240K, 150K, and 34K were obtained. These results demonstrated that circulating IgG antibody reactive with PA was present in patients with metastatic cancer of the prostate, and this in part was in complexed form with PA and was specifically reactive with ductal epithelial elements of the prostate.