In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours.
In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours.
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关于5-氨基酸酸中酸介导的光动力疗法对妇科肿瘤的潜在使用的体外研究。
DOI：
10.1038/bjc.1996.452
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发表时间：
1996-09
影响因子：
8.8
通讯作者：
Dickson, E F
中科院分区：
文献类型：
--
作者：
Rossi, F M;Campbell, D L;Pottier, R H;Kennedy, J C;Dickson, E F
结果报告各种妇科肿瘤细胞系的敏感性5-氨基乙酰丙酸诱导的原卟啉IX敏化光动力疗法（ALA-PDT）在体外。所有测试的细胞系积累ALA诱导的原卟啉IX（PpIX），并表现出对ALA-PDT的良好敏感性。通过荧光显微镜证实PpIX在线粒体中的定位。用透射电镜观察ALA-PDT后的亚细胞损伤。这种损伤最初局限于线粒体，随后观察到细胞膜和细胞核的损伤以及胞质内组织的完全丧失。卵巢癌多药耐药细胞系与其亲本细胞系之间ALA-PDT反应无明显差异。这些结果表明ALA-PDT在妇科恶性肿瘤的治疗中具有潜在的应用价值。
Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-PDT. Localisation of PpIX in the mitochondria was demonstrated by fluorescence microscopy. Subcellular damage following ALA-PDT was observed using transmission electron microscopy. This damage was localised initially to the mitochondria, with damage to membranes and the nucleus and complete loss of intracytoplasmic organisation being observed subsequently. There was no apparent difference in ALA-PDT response between a multidrug-resistant ovarian carcinoma cell line and its parent line. These results indicate that ALA-PDT has potential for application to therapy of gynaecological malignancies.